HCE Antibody (YA2959)
(Synonyms: HCE; HCE1; hCAP; CAP1A)HCE Antibody (YA2959) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HCE.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:20 |
Product Details
HCE Antibody (YA2959) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HCE.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 69 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 69 kDa
Entrez Gene: 8732 Human ; 24018 Mouse ; 313131 Rat
SwissProt: O60942 Human ; O55236 Mouse ;
OMIM: 603512 Human
Recombinant protein of human HCE
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
HCE is a Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle -
Isoforms & Post-Translational Modification
O60942 has 4 isomers: O60942-1: 68557 Da (predicted); O60942-2: 66042 Da (predicted); O60942-3: 52533 Da (predicted); O60942-4: 61705 Da (predicted).
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Subunit
Interacts with POLR2A (via C-terminus); this enhances guanylyltransferase activity. Binds (via GTase domain) to the elongating phosphorylated form of RNA polymerase II; can form direct interactions with the phosphorylated POLR2A C-terminal domain and indirect interactions via bound RNA (By similarity). Interacts with SUPT5H and RNMT. Interacts with HIV-1 Tat
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SwissProt ID
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Synonyms
HCE; HCE1; hCAP; CAP1A
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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