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SPON2 Antibody (YA2049)

(Synonyms: DIL1; DIL-1; MINDIN; M-SPONDIN)
Customer Review

Based on 1 Customer Validation

SPON2 Antibody (YA2049) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPON2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SPON2 Antibody (YA2049)
  • SPON2 Antibody (YA2049) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

SPON2 Antibody (YA2049) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPON2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human SPON2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104415

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SPON2 Antibody (YA2049)
    Western blot analysis of extracts from K562(lane 2(20ug)，NIH/3T3(lane 3(20ug) and C6(lane 4(20ug)using SPON2 Antibody (HY-P82304) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    SPON2 cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS)

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:Expressed in normal lung tissue but not in lung carcinoma cell lines

  • Subunit

    Monomer. Interacts with integrin

  • SwissProt ID

    Q9BUD6

  • Gene ID
    10417 [NCBI]
  • Synonyms

    DIL1; DIL-1; MINDIN; M-SPONDIN

  • Research Field

    Neuroscience

SPON2 Antibody (YA2049) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug)，NIH/3T3(lane 3(20ug) and C6(lane 4(20ug)using SPON2 Antibody (HY-P82304) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SPON2 Antibody (YA2049) Product Image
    1/16
  • Experimental Validation Results for SPON2 Antibody (YA2049)
  • SPON2 Antibody (YA2049) Product Image
  • Experimental Validation Results for SPON2 Antibody (YA2049)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug)，NIH/3T3(lane 3(20ug) and C6(lane 4(20ug)using SPON2 Antibody (HY-P82304) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SPON2 Antibody (YA2049)
  • SPON2 Antibody (YA2049) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug)，NIH/3T3(lane 3(20ug) and C6(lane 4(20ug)using SPON2 Antibody (HY-P82304) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SPON2 Antibody (YA2049) Product Image WB