Junctional Adhesion Molecule 1 Antibody (YA3025)(PBS only)

Cat. No.: HY-P83280A: Ficha de datos User Guide for Antibodies
FAQs
Technical Support

Junctional Adhesion Molecule 1 Antibody (YA3025) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Junctional Adhesion Molecule 1.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

* Seleccione Cantidad antes de agregar artículos.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

Junctional Adhesion Molecule 1 Antibody (YA3025) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Junctional Adhesion Molecule 1.

Host

Rabbit

Peso molecular

Predicted band size: 33 kDa;

Observed band size: 33 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y624

Gene ID

50848 [NCBI]

Immunogen

Recombinant protein of human Junctional Adhesion Molecule 1/JAM-A

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Pureza	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840); (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1; (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa

Subcellular Localization：Cell junction, tight junction; Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:It is expressed (at the protein level) in endothelial cells, epithelial cells, and leukocytes.

Isoforms & Post-Translational Modification：Q9Y624 has 2 isomers: Q9Y624-1: 32583 Da (predicted); Q9Y624-2: 27208 Da (predicted).
N-glycosylated;(Microbial infection) Cleaved by H.pylori virulence factor PqqE. Cleavage leads to altered tight junction functions

Subunit：Interacts with the ninth PDZ domain of MPDZ (PubMed:11489913). Interacts with the first PDZ domain of PARD3 (PubMed:11489913). The association between PARD3 and PARD6B probably disrupts this interaction (By similarity). Interacts with ITGAL (via I-domain) (PubMed:15528364). Interacts with CD151 (PubMed:35067832)

Research Field

Cardiovascular

Synonyms

F11R; JAM1; JCAM; Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; Platelet F11 receptor; Platelet adhesion molecule 1; PAM-1; CD321

Documentación

User Guide for Antibodies (1077 KB)