Integrin beta 6 Antibody (YA3122)
(Synonyms: AI1H; Integrin beta-6)Based on 1 publication(s) in Google Scholar
Integrin beta 6 Antibody (YA3122) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin beta 6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Publications Citing Use of MedChemExpress (MCE) Integrin beta 6 Antibody (YA3122)
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Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
Integrin beta 6 Antibody (YA3122) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin beta 6.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 97 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 86 kDa
Entrez Gene: 3694 Human ; 16420 Mouse ; 311061 Rat
SwissProt: P18564 Human ; Q9Z0T9 Mouse ; Q6AYF4 Rat
OMIM: 616221 Human
A synthetic peptide of human ITGB6
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Publications (1)
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Journal Impact Factor
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Most Recent
Verification Images
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Western blot analysis of extracts from C6 (lane2, 20μg), NIH/3T3 (lane3, 20μg), Hela (lane4, 20μg) and A431 (lane5, 20μg) using Integrin beta 6 Antibody (HY-P83377). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
Background
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Function
Integrin beta 6 is an Integrin alpha-V:beta-6 (ITGAV:ITGB6) is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalization of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV:ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. Integrin alpha-V:beta-6 (ITGAV:ITGB6) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation; (Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for Coxsackievirus A9 and Coxsackievirus B1; (Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for Herpes simplex virus-1/HHV-1[1][2][3][4][5][6].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Cell junction, focal adhesion
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Isoforms & Post-Translational Modification
P18564 has 2 isomers: P18564-1: 85936 Da (predicted); P18564-2: 74958 Da (predicted).
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Subunit
Heterodimer of an alpha and a beta subunit (PubMed:11807098, PubMed:17158881, PubMed:17545607). Interacts with FLNB (PubMed:11807098). Interacts with HAX1 (PubMed:17545607). ITGAV:ITGB6 interacts with FBN1 (PubMed:17158881). ITGAV:ITGB6 interacts with TGFB1 (PubMed:22278742, PubMed:28117447)
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SwissProt ID
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Synonyms
AI1H; Integrin beta-6
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Research Field
Signal Transduction
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
References
[1]. Jovanovic J, et al. alphaVbeta6 is a novel receptor for human fibrillin-1. Comparative studies of molecular determinants underlying integrin-rgd affinity and specificity. J Biol Chem. 2007 Mar 2;282(9):6743-51. [Content Brief]
[2]. Ramsay AG, et al. HS1-associated protein X-1 regulates carcinoma cell migration and invasion via clathrin-mediated endocytosis of integrin alphavbeta6. Cancer Res. 2007 Jun 1;67(11):5275-84. [Content Brief]
[3]. Annes JP, et al. Integrin alphaVbeta6-mediated activation of latent TGF-beta requires the latent TGF-beta binding protein-1. J Cell Biol. 2004 Jun 7;165(5):723-34. [Content Brief]
[4]. Wang R, et al. GARP regulates the bioavailability and activation of TGFβ. Mol Biol Cell. 2012 Mar;23(6):1129-39. [Content Brief]
[5]. Dong X, et al. Force interacts with macromolecular structure in activation of TGF-β. Nature. 2017 Feb 2;542(7639):55-59. [Content Brief]
[6]. Gianni T, et al. αvβ6- and αvβ8-integrins serve as interchangeable receptors for HSV gH/gL to promote endocytosis and activation of membrane fusion. PLoS Pathog. 2013;9(12):e1003806. [Content Brief]
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