SDHA Antibody (YA3948)
(Synonyms: FP; PGL5; SDH1; SDH2; SDHF; CMD1GG; MC2DN1; NDAXOA)SDHA Antibody (YA3948) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SDHA.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
WB, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
SDHA Antibody (YA3948) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SDHA.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 73 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 73 kDa
Purified recombinant fragment of human SDHA (AA: 516-665) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SDHA is a Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate. Can act as a tumor suppressor[1][2][3].
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Subcellular Localization
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
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Isoforms & Post-Translational Modification
P31040 has 3 isomers: P31040-1: 72692 Da (predicted); P31040-2: 67300 Da (predicted); P31040-3: 56650 Da (predicted).
Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation;Acetylated. Deacetylated by SIRT3 -
Subunit
Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD (PubMed:37098072). Interacts with SDHAF2/SDH5; interaction is required for FAD attachment (PubMed:19628817, PubMed:32887801). Interacts with TRAP1 (PubMed:23747254). Interacts with LACC1 (PubMed:28593945)
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SwissProt ID
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Synonyms
FP; PGL5; SDH1; SDH2; SDHF; CMD1GG; MC2DN1; NDAXOA
Documentation
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