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PAIP1 Antibody (YA1528)

(Synonyms: poly(A) binding protein interacting protein 1)
Customer Review

Based on 1 Customer Validation

PAIP1 Antibody (YA1528) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAIP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • PAIP1 Antibody (YA1528) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

PAIP1 Antibody (YA1528) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAIP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PAIP1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103887

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), Hela (lane 2, 20 μg), and A549 (lane 3, 20 μg) using PAIP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    Immunohistochemical analysis of paraffin-embedded rat vagina tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    Immunohistochemical analysis of paraffin-embedded rat fallopian tube tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    Immunohistochemical analysis of paraffin-embedded rat ovary tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    Immunohistochemical analysis of paraffin-embedded rat hippocampal formation tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    Immunocytochemistry analysis of Hela cells labeling PAIP1 with PAIP1 Antibody (HY-P81783) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PAIP1 Antibody (HY-P81783) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    Immunocytochemistry analysis of Hela cells labeling PAIP1 with PAIP1 Antibody (HY-P81783) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PAIP1 Antibody (HY-P81783) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain; (Microbial infection) Upon interaction with SARS coronavirus SARS-CoV NSP3 protein, plays an important role in viral protein synthesis

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    Q9H074 has 3 isomers: Q9H074-1: 53525 Da (predicted); Q9H074-2: 45631 Da (predicted); Q9H074-3: 42007 Da (predicted).

  • Subunit

    Interacts with the RRM1-RRM2 and C-terminus regions of PABPC1 in a 1:1 stoichiometry. Interacts with EIF4A

  • SwissProt ID

    Q9H074

  • Gene ID
    10605 [NCBI]
  • Synonyms

    poly(A) binding protein interacting protein 1

  • Research Field

    Epigenetics and Nuclear Signaling

PAIP1 Antibody (YA1528) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), Hela (lane 2, 20 μg), and A549 (lane 3, 20 μg) using PAIP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat spleen tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat vagina tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat fallopian tube tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat ovary tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat hippocampal formation tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PAIP1 with PAIP1 Antibody (HY-P81783) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PAIP1 Antibody (HY-P81783) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PAIP1 with PAIP1 Antibody (HY-P81783) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PAIP1 Antibody (HY-P81783) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PAIP1 Antibody (YA1528) Product Image
    1/16
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • PAIP1 Antibody (YA1528) Product Image
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), Hela (lane 2, 20 μg), and A549 (lane 3, 20 μg) using PAIP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat spleen tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat vagina tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat fallopian tube tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat ovary tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat hippocampal formation tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PAIP1 with PAIP1 Antibody (HY-P81783) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PAIP1 Antibody (HY-P81783) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PAIP1 with PAIP1 Antibody (HY-P81783) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PAIP1 Antibody (HY-P81783) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • Experimental Validation Results for PAIP1 Antibody (YA1528)
  • PAIP1 Antibody (YA1528) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), Hela (lane 2, 20 μg), and A549 (lane 3, 20 μg) using PAIP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PAIP1 Antibody (YA1528) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAIP1 Antibody (YA1528) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat vagina tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAIP1 Antibody (YA1528) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat fallopian tube tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAIP1 Antibody (YA1528) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAIP1 Antibody (YA1528) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAIP1 Antibody (YA1528) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat hippocampal formation tissue using PAIP1 Antibody (HY-P81783, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAIP1 Antibody (YA1528) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PAIP1 with PAIP1 Antibody (HY-P81783) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PAIP1 Antibody (HY-P81783) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PAIP1 Antibody (YA1528) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PAIP1 with PAIP1 Antibody (HY-P81783) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PAIP1 Antibody (HY-P81783) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PAIP1 Antibody (YA1528) Product Image WB
  • PAIP1 Antibody (YA1528) Product Image IHC-P
  • PAIP1 Antibody (YA1528) Product Image IHC-P
  • PAIP1 Antibody (YA1528) Product Image IHC-P
  • PAIP1 Antibody (YA1528) Product Image IHC-P
  • PAIP1 Antibody (YA1528) Product Image IHC-P
  • PAIP1 Antibody (YA1528) Product Image IHC-P
  • PAIP1 Antibody (YA1528) Product Image ICC
  • PAIP1 Antibody (YA1528) Product Image ICC