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HDAC9 Antibody (YA2967)

(Synonyms: Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR)
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Based on 1 Customer Validation

HDAC9 Antibody (YA2967) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC9.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HDAC9 Antibody (YA2967)
  • HDAC9 Antibody (YA2967) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

HDAC9 Antibody (YA2967) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC9.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 111 kDa
Species Reactivity Database

Entrez Gene: 9734 Human

SwissProt: Q9UKV0 Human

Immunogen

A synthetic peptide of human HDAC9

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105336

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HDAC9 Antibody (YA2967)
    Western blot analysis of extracts from Hela(lane 2(20ug) ，HL-60(lane 3(20ug) and HEK293(lane 4(20ug) using HDAC9 Antibody (HY-P83222) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    HDAC9 is a class IIa histone deacetylase linked to cardiac muscle development, bone formation, adipocyte differentiation, innate immunity, and cancer biology[1]. Mechanistically, HDAC9 forms a myogenic negative-feedback circuit because MEF2 directly induces HDAC9, while HDAC9 associates with MEF2 proteins and suppresses their transcriptional activity[2]. In ischemic disease models, HDAC9 promotes brain ischemic injury through IκBα/NF-κB and MAPK signaling, and HDAC9 inhibition reduces inflammation-linked injury[3]. Stroke-focused evidence further supports HDAC9 inhibition as a treatment concept, including reduced infarct volume and improved neurological function in HDAC9-knockout mice after ischemic reperfusion injury[4]. In cancer models, HDAC9 regulates breast cancer cell proliferation and modifies cellular responses to histone deacetylase inhibitors[5]. Compared with related class IIa isoforms HDAC4, HDAC5, and HDAC7, HDAC9 should be analyzed as an isoform-specific disease regulator while class IIa-selective inhibitors remain useful experimental probes across this subgroup[6].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level)

  • Isoforms & Post-Translational Modification

    Q9UKV0 has 11 isomers: Q9UKV0-1: 111297 Da (predicted); Q9UKV0-2: 101805 Da (predicted); Q9UKV0-3: 65887 Da (predicted); Q9UKV0-4: 97471 Da (predicted); Q9UKV0-5: 117208 Da (predicted); Q9UKV0-6: 112451 Da (predicted); Q9UKV0-7: 117577 Da (predicted); Q9UKV0-8: 65508 Da (predicted); Q9UKV0-9: 60761 Da (predicted); Q9UKV0-10: 62852 Da (predicted); Q9UKV0-11: 57092 Da (predicted).
    Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import;Sumoylated

  • Subunit

    Homodimer. Interacts with CTBP1.

  • SwissProt ID

    Q9UKV0

  • Gene ID
    9734 [NCBI]
  • Synonyms

    Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Yang C, et al. Histone deacetylase (HDAC) 9: versatile biological functions and emerging roles in human cancer. Cell Oncol (Dordr). 2021 Oct;44(5):997-1017. [Content Brief]

[2]. Haberland M, et al. Regulation of HDAC9 gene expression by MEF2 establishes a negative-feedback loop in the transcriptional circuitry of muscle differentiation. Mol Cell Biol. 2007 Jan;27(2):518-25. [Content Brief]

[3]. Lu S, et al. HDAC9 promotes brain ischemic injury by provoking IκBα/NF-κB and MAPKs signaling pathways. Biochem Biophys Res Commun. 2018 Sep 10;503(3):1322-1329. [Content Brief]

[4]. Markus HS. HDAC9 Inhibition as a Novel Treatment for Stroke. Stroke. 2023 Dec;54(12):3182-3189. doi: 10.1161/STROKEAHA.123.044862. Epub 2023 Nov 9. PMID: 37942644. et al. HDAC9 Inhibition as a Novel Treatment for Stroke. Stroke. 2023 Dec;54(12):3182-3189. [Content Brief]

[5]. Lapierre M, et al. Histone deacetylase 9 regulates breast cancer cell proliferation and the response to histone deacetylase inhibitors. Oncotarget. 2016 Apr 12;7(15):19693-708. [Content Brief]

[6]. Lobera M, et al. Selective class IIa histone deacetylase inhibition via a nonchelating zinc-binding group. Nat Chem Biol. 2013 May;9(5):319-25. [Content Brief]

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HDAC9 Antibody (YA2967) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) ，HL-60(lane 3(20ug) and HEK293(lane 4(20ug) using HDAC9 Antibody (HY-P83222) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HDAC9 Antibody (YA2967) Product Image
    1/16
  • Experimental Validation Results for HDAC9 Antibody (YA2967)
  • HDAC9 Antibody (YA2967) Product Image
  • Experimental Validation Results for HDAC9 Antibody (YA2967)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) ，HL-60(lane 3(20ug) and HEK293(lane 4(20ug) using HDAC9 Antibody (HY-P83222) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HDAC9 Antibody (YA2967)
  • HDAC9 Antibody (YA2967) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) ，HL-60(lane 3(20ug) and HEK293(lane 4(20ug) using HDAC9 Antibody (HY-P83222) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HDAC9 Antibody (YA2967) Product Image WB