HDAC9 Antibody (YA2967)

Cat. No.: HY-P83222: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

HDAC9 Antibody (YA2967) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC9.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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HDAC9 Antibody (YA2967) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

HDAC9 Antibody (YA2967) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC9.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 111 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9UKV0

Gene ID

9734 [NCBI]

Immunogen

A synthetic peptide of human HDAC9

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela(lane 2(20ug) ，HL-60(lane 3(20ug) and HEK293(lane 4(20ug) using HDAC9 Antibody (HY-P83222) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription; Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level)

Isoforms & Post-Translational Modification：Q9UKV0 has 11 isomers: Q9UKV0-1: 111297 Da (predicted); Q9UKV0-2: 101805 Da (predicted); Q9UKV0-3: 65887 Da (predicted); Q9UKV0-4: 97471 Da (predicted); Q9UKV0-5: 117208 Da (predicted); Q9UKV0-6: 112451 Da (predicted); Q9UKV0-7: 117577 Da (predicted); Q9UKV0-8: 65508 Da (predicted); Q9UKV0-9: 60761 Da (predicted); Q9UKV0-10: 62852 Da (predicted); Q9UKV0-11: 57092 Da (predicted).
Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import;Sumoylated

Subunit：Homodimer. Interacts with CTBP1.

RRID

AB_3105336

Database

Entrez Gene: 9734 Human

SwissProt: Q9UKV0 Human

OMIM: 620457 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

HDAC9 Antibody (YA2967) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HDAC9 Antibody (YA2967)

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