BIN1 Antibody (YA2275)(PBS only)

Cat. No.: HY-P82530A: Ficha de datos User Guide for Antibodies
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BIN1 Antibody (YA2275) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BIN1.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

BIN1 Antibody (YA2275) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BIN1.

Host

Rabbit

Peso molecular

Predicted band size: 65 kDa;

Observed band size: 45-80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O00499

Gene ID

274 [NCBI]

Immunogen

Recombinant protein of human BIN1 aa445-593.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Pureza	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:24755653). Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:27179792). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation (PubMed:8782822). Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro (PubMed:28893863)

Subcellular Localization：Nucleus; Cytoplasm; Endosome; Cell membrane, sarcolemma, T-tubule; Cytoplasm

Expression：
Tissue_specificity:Ubiquitous. Highest expression in the brain and muscle (PubMed:9182667) . Expressed in oligodendrocytes (PubMed:27488240) . Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter (PubMed:27488240) . Isoform BIN1 is widely expressed with highest expression in skeletal muscle

Isoforms & Post-Translational Modification：O00499 has 11 isomers: O00499-1: 64699 Da (predicted); O00499-2: 56499 Da (predicted); O00499-3: 55175 Da (predicted); O00499-4: 51737 Da (predicted); O00499-5: 59937 Da (predicted); O00499-6: 53020 Da (predicted); O00499-7: 48258 Da (predicted); O00499-8: 50185 Da (predicted); O00499-9: 45563 Da (predicted); O00499-10: 47491 Da (predicted); O00499-11: 54948 Da (predicted).
Phosphorylated by protein kinase C

Subunit：Heterodimer with AMPH (By similarity). Binds SH3GLB1 (By similarity). Interacts (via SH3 domain) with DNM1. Interacts with SYNJ1 (By similarity). Interacts (via SH3 domain) with DNM2 (PubMed:17676042). Isoform IIA interacts with CLTC.

Research Field

Cell Biology

Synonyms

BIN1; AMPHL; Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/a549-cell.html [Content Brief]