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LGR5 Antibody (YA1842)

(Synonyms: FEX; GPR49; GPR67; GRP49; LGR5; HG38; 8)
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Based on 1 Customer Validation

LGR5 Antibody (YA1842) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LGR5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LGR5 Antibody (YA1842)
  • LGR5 Antibody (YA1842) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50 1:50-1:100

Product Details

Description

LGR5 Antibody (YA1842) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LGR5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 100 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human LGR5/GPR49 aa818-907.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104026

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LGR5 Antibody (YA1842)
    Western blot analysis of extracts from HepG2(lane 2(20μg), HCT116(lane 3(20μg), HeLa(lane 4(20μg), SH-SY5Y(lane 5(20μg) using LGR5 Antibody (HY-P82097). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    LGR5 receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver

  • Isoforms & Post-Translational Modification

    O75473 has 3 isomers: O75473-1: 99998 Da (predicted); O75473-2: 97404 Da (predicted); O75473-3: 92006 Da (predicted).

  • Subunit

    Identified in a complex composed of RNF43, LGR5 and RSPO1 (PubMed:21693646, PubMed:21727895, PubMed:21909076, PubMed:22815884, PubMed:23756651, PubMed:23809763). Also interacts with other R-spondin ligands, including RSPO2, RSPO3 and RSPO4 (PubMed:21693646, PubMed:21727895, PubMed:21909076, PubMed:29769720)

  • SwissProt ID

    O75473

  • Gene ID
    8549 [NCBI]
  • Synonyms

    FEX; GPR49; GPR67; GRP49; LGR5; HG38; 8

  • Research Field

    Signal Transduction

LGR5 Antibody (YA1842) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20μg), HCT116(lane 3(20μg), HeLa(lane 4(20μg), SH-SY5Y(lane 5(20μg) using LGR5 Antibody (HY-P82097). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • LGR5 Antibody (YA1842) Product Image
    1/16
  • Experimental Validation Results for LGR5 Antibody (YA1842)
  • LGR5 Antibody (YA1842) Product Image
  • Experimental Validation Results for LGR5 Antibody (YA1842)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20μg), HCT116(lane 3(20μg), HeLa(lane 4(20μg), SH-SY5Y(lane 5(20μg) using LGR5 Antibody (HY-P82097). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LGR5 Antibody (YA1842)
  • LGR5 Antibody (YA1842) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20μg), HCT116(lane 3(20μg), HeLa(lane 4(20μg), SH-SY5Y(lane 5(20μg) using LGR5 Antibody (HY-P82097). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • LGR5 Antibody (YA1842) Product Image WB