LGR5 Antibody (YA1842)

製品番号: HY-P82097: データシート SDS COA User Guide for Antibodies
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LGR5 Antibody (YA1842) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LGR5.

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容量	価格（税別）	在庫状況	数量
20 μL	$144	在庫あり	Estimated Time of Arrival: December 31
50 μL	$200	在庫あり	Estimated Time of Arrival: December 31
100 μL	$325	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

LGR5 Antibody (YA1842) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LGR5.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 100 kDa;

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O75473

Gene ID

8549 [NCBI]

Immunogen

A synthesized peptide derived from human LGR5/GPR49 aa818-907.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	純度	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2(lane 2(20μg), HCT116(lane 3(20μg), HeLa(lane 4(20μg), SH-SY5Y(lane 5(20μg) using LGR5 Antibody (HY-P82097). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver

Isoforms & Post-Translational Modification：O75473 has 3 isomers: O75473-1: 99998 Da (predicted); O75473-2: 97404 Da (predicted); O75473-3: 92006 Da (predicted).

Subunit：Identified in a complex composed of RNF43, LGR5 and RSPO1 (PubMed:21693646, PubMed:21727895, PubMed:21909076, PubMed:22815884, PubMed:23756651, PubMed:23809763). Also interacts with other R-spondin ligands, including RSPO2, RSPO3 and RSPO4 (PubMed:21693646, PubMed:21727895, PubMed:21909076, PubMed:29769720)

RRID

AB_3104026

Database

Entrez Gene: 8549 Human ; 14160 Mouse ; 299802 Rat

SwissProt: O75473 Human ; Q9Z1P4 Mouse ;

OMIM: 606667 Human

Research Field

Signal Transduction

Synonyms

FEX; GPR49; GPR67; GRP49; LGR5; HG38; 8

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)