GABA A Receptor beta 2 Antibody
(Synonyms: GABA, GABA(A) receptor beta 2, GABA(A) receptor subunit beta-2, GABA-A receptor, beta-2 polypeptide, GABRB2, Gamma aminobutyric acid (GABA) A receptor, beta 2, Gamma aminobutyric acid A receptor beta 2, Gamma-aminobutyric acid receptor subunit beta-2, Gamma-aminobutyric-acid receptor subunit beta-2, GBRB2_HUMAN)GABA A Receptor beta 2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to GABA A Receptor beta 2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:200 | 1:500-1000 |
Product Details
GABA A Receptor beta 2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to GABA A Receptor beta 2.
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Host Rabbit
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Clonality polyclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 59 kDa;
Synthetic peptide of human GABRB2 (aa 350-400).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GABA A Receptor beta 2 is a Beta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (By similarity). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (By similarity). GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation. Extrasynaptic beta-2 receptors contribute to the tonic GABAergic inhibition (By similarity). Beta-containing GABAARs can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness (By similarity)[1][2][3][4][5][6].
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Subcellular Localization
Postsynaptic cell membrane; Cell membrane; Cytoplasmic vesicle membrane
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Expression
Tissue_Specificity: Isoform 1 and isoform 2 show reduced expression in schizophrenic brain. Isoform 3 shows increased expression in schizophrenic and bipolar disorder brains while isoform 4 shows reduced expression. -
Isoforms & Post-Translational Modification
GABA A Receptor beta 2 has 4 isoforms, P47870-2: amino acid length is 512, molecular weight is 59150 Da (predicted); P47870-1: amino acid length is 474, molecular weight is 54606 Da (predicted); P47870-3: amino acid length is 313, molecular weight is 36086 Da (predicted); P47870-4: amino acid length is 372, molecular weight is 42741 Da (predicted).
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Subunit
Heteropentamer, formed by a combination of alpha (GABRA1-6), beta (GABRB1-3), gamma (GABRG1-3), delta (GABRD), epsilon (GABRE), rho (GABRR1-3), pi (GABRP) and theta (GABRQ) chains, each subunit exhibiting distinct physiological and pharmacological properties.
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SwissProt ID
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Synonyms
GABA, GABA(A) receptor beta 2, GABA(A) receptor subunit beta-2, GABA-A receptor, beta-2 polypeptide, GABRB2, Gamma aminobutyric acid (GABA) A receptor, beta 2, Gamma aminobutyric acid A receptor beta 2, Gamma-aminobutyric acid receptor subunit beta-2, Gamma-aminobutyric-acid receptor subunit beta-2, GBRB2_HUMAN
Documentation
References
[1]. Zhao C, et al. Alternative-splicing in the exon-10 region of GABA(A) receptor beta(2) subunit gene: relationships between novel isoforms and psychotic disorders. PLoS One. 2009 Sep 18;4(9):e6977. [Content Brief]
[2]. Ishii A, et al. A de novo missense mutation of GABRB2 causes early myoclonic encephalopathy. J Med Genet. 2017 Mar;54(3):202-211. [Content Brief]
[3]. Zhu S, et al. Structure of a human synaptic GABA(A) receptor. Nature. 2018 Jul;559(7712):67-72. [Content Brief]
[4]. Hadingham KL, et al. Role of the beta subunit in determining the pharmacology of human gamma-aminobutyric acid type A receptors. Mol Pharmacol. 1993 Dec;44(6):1211-8. [Content Brief]
[5]. Fuchs C, et al. GABA(A) receptors can initiate the formation of functional inhibitory GABAergic synapses. Eur J Neurosci. 2013 Oct;38(8):3146-58. [Content Brief]
[6]. Brown LE, et al. Inhibitory synapse formation in a co-culture model incorporating GABAergic medium spiny neurons and HEK293 cells stably expressing GABAA receptors. J Vis Exp. 2014 Nov 14;(93):e52115. [Content Brief]
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