Copy Link Print Information

Topoisomerase II alpha Antibody (YA3544)

(Synonyms: DNA topoisomerase II; DNA topoisomerase II-alpha; TOP2; TP2A; alpha isozyme, Topoisomerase II alpha)
Customer Review

Based on 1 Customer Validation

Topoisomerase II alpha Antibody (YA3544) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Topoisomerase II alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
  • Topoisomerase II alpha Antibody (YA3544) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

Topoisomerase II alpha Antibody (YA3544) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Topoisomerase II alpha.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 174 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 174 kDa
Species Reactivity Database
Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102783

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
    Western blot analysis of extracts from Hela (lane2(20μg), Jurkat (lane3(20μg), NIH/3T3 (lane4(20μg) and HT-29 (lane5(20μg) using Topoisomerase II alpha Antibody (HY-P80980). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using TOP2A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using TOP2A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Topoisomerase II alpha is key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation[1][2][3][4].

  • Subcellular Localization

    Cytoplasm; Nucleus, nucleoplasm; Nucleus; Nucleus, nucleolus

  • Expression


    Tissue_specificity:This gene is expressed in the tonsils, spleen, lymph nodes, thymus, skin, pancreas, testes, colon, kidneys, liver, brain, and lungs (PubMed:9155056) . It is also found in high-grade lymphoma, squamous cell carcinoma of the lung, and seminoma (PubMed:9155056) .

  • Isoforms & Post-Translational Modification

    P11388 has 4 isomers: P11388-1: 174385 Da (predicted); P11388-2: 177501 Da (predicted); P11388-3: 178712 Da (predicted); P11388-4: 182681 Da (predicted).
    Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation;(Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1; leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks;SUMOylated

  • Subunit

    Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity (PubMed:18790802, PubMed:20457750). Interacts with DHX9; this interaction occurs in a E2 enzyme UBE2I- and RNA-dependent manner, negatively regulates DHX9-mediated double-stranded DNA and RNA duplex helicase activity and stimulates TOP2A-mediated supercoiled DNA relaxation activity (PubMed:12711669). Interacts with HNRNPU (via C-terminus); this interaction protects the topoisomerase TOP2A from degradation and positively regulates the relaxation of supercoiled DNA in a RNA-dependent manner (By similarity). Interacts with MCM3AP isoform GANP (PubMed:23652018). Interacts with ERCC6 (PubMed:26030138). Interacts with PLSCR1 (PubMed:17567603). Interacts with GCNA; this interaction allows the resolution of topoisomerase II (TOP2A) DNA-protein cross-links (By similarity). Interacts with POL1RA/RPA1 (via dock II) and UBTF in the context of Pol I complex; may assist Pol I transcription initiation by releasing supercoils occurring during DNA unwinding. Interacts with TPRN; TPRN interacts with a number of DNA damage response proteins, is recruited to sites of DNA damage and may play a role in DNA damage repair (PubMed:23213405)

  • SwissProt ID

    P11388

  • Gene ID
    7153 [NCBI]
  • Synonyms

    DNA topoisomerase II; DNA topoisomerase II-alpha; TOP2; TP2A; alpha isozyme, Topoisomerase II alpha

Topoisomerase II alpha Antibody (YA3544) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), Jurkat (lane3(20μg), NIH/3T3 (lane4(20μg) and HT-29 (lane5(20μg) using Topoisomerase II alpha Antibody (HY-P80980). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using TOP2A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using TOP2A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Topoisomerase II alpha Antibody (YA3544) Product Image
    1/16
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
  • Topoisomerase II alpha Antibody (YA3544) Product Image
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), Jurkat (lane3(20μg), NIH/3T3 (lane4(20μg) and HT-29 (lane5(20μg) using Topoisomerase II alpha Antibody (HY-P80980). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using TOP2A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using TOP2A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
  • Experimental Validation Results for Topoisomerase II alpha Antibody (YA3544)
  • Topoisomerase II alpha Antibody (YA3544) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), Jurkat (lane3(20μg), NIH/3T3 (lane4(20μg) and HT-29 (lane5(20μg) using Topoisomerase II alpha Antibody (HY-P80980). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • Topoisomerase II alpha Antibody (YA3544) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using TOP2A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Topoisomerase II alpha Antibody (YA3544) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using TOP2A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Topoisomerase II alpha Antibody (YA3544) Product Image WB
  • Topoisomerase II alpha Antibody (YA3544) Product Image IHC-P
  • Topoisomerase II alpha Antibody (YA3544) Product Image IHC-P