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Cat. No.: HY-P810435

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Gemin 2 Antibody (YA9747)

Name: Gemin 2 Antibody (YA9747)
Brand: MedChemExpress
SKU: HY-P810435
Price: 120.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: Component of gems 2, Gem (nuclear organelle) associated protein 2, Gem associated protein 2, GEMI2_HUMAN, Gemin-2, gemin2, SIP 1, SIP-1, SIP1, SIP1 delta)

Customer Review

Based on 1 Customer Validation

Gemin 2 Antibody (YA9747) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to Gemin 2.

For research use only. We do not sell to patients.

Host:
Rabbit
Isotype:
IgG
Application:
WB, ICC/IF, FC, IP
Reactivity :
Human, Mouse, Rat

Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Conjugation:
Non-conjugated

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications
Product Details
Product Properties
- Appearance
- Formulation
- Concentration
- Storage & Stability
- Shipping
Background
Documentation
References
Help & FAQs
Classifications
Bioz

Applications

Application	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	FC FC: Flow Cytometry	IP IP: Immunoprecipitation
Dilution Ratio	1:1000	1:100	1:1000	1-2μg/sample

Product Details

Description

Gemin 2 Antibody (YA9747) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to Gemin 2.

Host Rabbit
Clonality Recombinant,Monoclonal
Species Reactivity
Human, Mouse, Rat
Observed Molecular Weight

Observed band size: 32 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 32 kDa

Immunogen

Synthetic peptide of Human Gemin 2 aa 1-100.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

Appearance

Solution
Formulation

Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration

Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Background

Function

Gemin 2 is a The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound^[1]^[2]^[3]^[4]^[5].
Subcellular Localization

Nucleus, gem; Cytoplasm
Isoforms & Post-Translational Modification

Gemin 2 has 5 isoforms, O14893-1: amino acid length is 280, molecular weight is 31585 Da (predicted); O14893-2: amino acid length is 265, molecular weight is 29931 Da (predicted); O14893-3: amino acid length is 250, molecular weight is 28155 Da (predicted); O14893-4: amino acid length is 44, molecular weight is 4826 Da (predicted); O14893-5: amino acid length is 269, molecular weight is 30358 Da (predicted).
Subunit

Monomer.
SwissProt ID

O14893
Synonyms

Component of gems 2, Gem (nuclear organelle) associated protein 2, Gem associated protein 2, GEMI2_HUMAN, Gemin-2, gemin2, SIP 1, SIP-1, SIP1, SIP1 delta

Documentation

Select Batch:

Data Sheet (260 KB)
SDS (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
User Guide for Antibodies (1077 KB)

References

[1]. Chari A, et al. An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs. Cell. 2008 Oct 31;135(3):497-509. [Content Brief]

[2]. Liu Q, et al. The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell. 1997 Sep 19;90(6):1013-21. [Content Brief]

[3]. Yi H, et al. Negative cooperativity between Gemin2 and RNA provides insights into RNA selection and the SMN complex's release in snRNP assembly. Nucleic Acids Res. 2020 Jan 24;48(2):895-911. [Content Brief]

[4]. Golembe TJ, et al. Specific sequence features, recognized by the SMN complex, identify snRNAs and determine their fate as snRNPs. Mol Cell Biol. 2005 Dec;25(24):10989-1004. [Content Brief]

[5]. Zhang R, et al. Structure of a key intermediate of the SMN complex reveals Gemin2's crucial function in snRNP assembly. Cell. 2011 Aug 5;146(3):384-95. [Content Brief]