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ACTL6A Antibody (YA2684)

(Synonyms: ACTL6A; BAF53; BAF53A; INO80K; Actin-like protein 6A; 53 kDa BRG1-associated factor A; Actin-related protein Baf53a; ArpNbeta; BRG1-associated factor 53A; BAF53A; INO80 complex subunit K)
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Based on 1 Customer Validation

ACTL6A Antibody (YA2684) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACTL6A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ACTL6A Antibody (YA2684)
  • ACTL6A Antibody (YA2684) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

ACTL6A Antibody (YA2684) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACTL6A.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database

Entrez Gene: 86 Human

SwissProt: O96019 Human

Immunogen

A synthetic peptide of human ACTL6A

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105058

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ACTL6A Antibody (YA2684)
    Western blot analysis was performed on extracts from Ramos (lane 1, 20 μg), K562 (lane 2, 20 μg), HeLa (lane 3, 20 μg), Jurkat (lane 4, 20 μg), MCF-7 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using ACTL6A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ACTL6A is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    O96019 has 2 isomers: O96019-1: 47461 Da (predicted); O96019-2: 43236 Da (predicted).

  • Subunit

    Component of numerous complexes with chromatin remodeling and histone acetyltransferase activity.

  • SwissProt ID

    O96019

  • Gene ID
    86 [NCBI]
  • Synonyms

    ACTL6A; BAF53; BAF53A; INO80K; Actin-like protein 6A; 53 kDa BRG1-associated factor A; Actin-related protein Baf53a; ArpNbeta; BRG1-associated factor 53A; BAF53A; INO80 complex subunit K

  • Research Field

    Signal Transduction

ACTL6A Antibody (YA2684) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Ramos (lane 1, 20 μg), K562 (lane 2, 20 μg), HeLa (lane 3, 20 μg), Jurkat (lane 4, 20 μg), MCF-7 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using ACTL6A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ACTL6A Antibody (YA2684) Product Image
    1/16
  • Experimental Validation Results for ACTL6A Antibody (YA2684)
  • ACTL6A Antibody (YA2684) Product Image
  • Experimental Validation Results for ACTL6A Antibody (YA2684)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Ramos (lane 1, 20 μg), K562 (lane 2, 20 μg), HeLa (lane 3, 20 μg), Jurkat (lane 4, 20 μg), MCF-7 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using ACTL6A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ACTL6A Antibody (YA2684)
  • ACTL6A Antibody (YA2684) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Ramos (lane 1, 20 μg), K562 (lane 2, 20 μg), HeLa (lane 3, 20 μg), Jurkat (lane 4, 20 μg), MCF-7 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using ACTL6A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ACTL6A Antibody (YA2684) Product Image WB