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Syntaxin 1A Antibody (YA2001)

(Synonyms: STX1A; STX1; Syntaxin-1A; Neuron-specific antigen HPC-1)
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Based on 1 Customer Validation

Syntaxin 1A Antibody (YA2001) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syntaxin 1A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
  • Syntaxin 1A Antibody (YA2001) Product Image
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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50 1:50-1:100

Product Details

Description

Syntaxin 1A Antibody (YA2001) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syntaxin 1A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human STX1A aa1-39.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104368

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
    Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) and SH-SY5Y(lane 3(40ug) using Syntaxin 1A Antibody (HY-P82256) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Syntaxin 1A Antibody (HY-P82256, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Syntaxin 1A plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1)[1][2].

  • Subcellular Localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein; Synapse, synaptosome; Cell membrane; Secreted

  • Expression


    Tissue_specificity:Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex; Expressed in heart, liver, fat, skeletal muscle, kidney and brain

  • Isoforms & Post-Translational Modification

    Q16623 has 3 isomers: Q16623-1: 33023 Da (predicted); Q16623-2: 29632 Da (predicted); Q16623-3: 28995 Da (predicted).
    Phosphorylated by CK2 (By similarity). Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1;Sumoylated, sumoylation is required for regulation of synaptic vesicle endocytosis

  • Subunit

    Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex constitutes the basic catalytic machinery of the complex neurotransmitter release apparatus (PubMed:26635000). The SNARE complex interacts with CPLX1 (By similarity). Interacts with STXBP1 (PubMed:12730201, PubMed:26635000). The interaction with STXBP1 promotes assembly of the SNARE complex (By similarity). Interacts (via C-terminus) with KCNB1 (via C-terminus); the interaction increases in a calcium-dependent manner and induces a pore-independent enhancement of exocytosis in neuroendocrine cells, cH2O2affin cells, pancreatic beta cells and from the soma of dorsal root ganglia (DRG) neurons (By similarity). Interacts with SYTL4 (By similarity). Interacts with STXBP6 (By similarity). Interacts with PLCL1 (via C2 domain) (By similarity). Interacts witH2O2OF (By similarity). Interacts with LGI3 (By similarity). Interacts (via the H3 domain) with SLC6A4 (via the N-terminus); this interaction regulates SLC4A6 channel conductance in thalamocortical neurons (By similarity). Interacts with SYT6 and SYT8; the interaction is Ca(2+)-dependent (By similarity). Interacts with VAMP8 (PubMed:12130530). Interacts with SNAP23 (PubMed:12130530). Interacts with VAPA and SYBU (PubMed:15459722). Interacts with PRRT2 (By similarity). Interacts with SEPT8 (By similarity). Interacts with STXBP5L (By similarity). Interacts with synaptotagmin-1/SYT1 (By similarity). Interacts with SEPTIN5; in the cerebellar cortex (By similarity). Interacts with SEPTIN4; in the striatum (By similarity)

  • SwissProt ID

    Q16623

  • Gene ID
    6804 [NCBI]
  • Synonyms

    STX1A; STX1; Syntaxin-1A; Neuron-specific antigen HPC-1

  • Research Field

    Neuroscience

[1]. Craig TJ, et al. SUMOylation of Syntaxin1A regulates presynaptic endocytosis. Sci Rep. 2015 Dec 4;5:17669. [Content Brief]

[2]. Rodríguez F, et al. Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis. J Biol Chem. 2012 Dec 21;287(52):43825-39. [Content Brief]

Syntaxin 1A Antibody (YA2001) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) and SH-SY5Y(lane 3(40ug) using Syntaxin 1A Antibody (HY-P82256) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Syntaxin 1A Antibody (HY-P82256, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Syntaxin 1A Antibody (YA2001) Product Image
    1/16
  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
  • Syntaxin 1A Antibody (YA2001) Product Image
  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) and SH-SY5Y(lane 3(40ug) using Syntaxin 1A Antibody (HY-P82256) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Syntaxin 1A Antibody (HY-P82256, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
  • Experimental Validation Results for Syntaxin 1A Antibody (YA2001)
  • Syntaxin 1A Antibody (YA2001) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) and SH-SY5Y(lane 3(40ug) using Syntaxin 1A Antibody (HY-P82256) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Syntaxin 1A Antibody (YA2001) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Syntaxin 1A Antibody (HY-P82256, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Syntaxin 1A Antibody (YA2001) Product Image WB
  • Syntaxin 1A Antibody (YA2001) Product Image FC