Syntaxin 1A Antibody (YA2001)

Cat. No.: HY-P82256: Data Sheet COA
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Syntaxin 1A Antibody (YA2001) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syntaxin 1A.

For research use only. We do not sell to patients.

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10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
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Syntaxin 1A Antibody (YA2001) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Syntaxin 1A Antibody (YA2001) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syntaxin 1A.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 33 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q16623

Gene ID

6804 [NCBI]

Immunogen

A synthesized peptide derived from human STX1A aa1-39.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) and SH-SY5Y(lane 3(40ug) using Syntaxin 1A Antibody (HY-P82256) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed:26635000). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm (PubMed:23091057). Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (By similarity)

Subcellular Localization：Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein; Synapse, synaptosome; Cell membrane; Secreted

Expression：
Tissue_specificity:Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex; Expressed in heart, liver, fat, skeletal muscle, kidney and brain

Isoforms & Post-Translational Modification：Q16623 has 3 isomers: Q16623-1: 33023 Da (predicted); Q16623-2: 29632 Da (predicted); Q16623-3: 28995 Da (predicted).
Phosphorylated by CK2 (By similarity). Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1;Sumoylated, sumoylation is required for regulation of synaptic vesicle endocytosis

Subunit：Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex constitutes the basic catalytic machinery of the complex neurotransmitter release apparatus (PubMed:26635000). The SNARE complex interacts with CPLX1 (By similarity). Interacts with STXBP1 (PubMed:12730201, PubMed:26635000). The interaction with STXBP1 promotes assembly of the SNARE complex (By similarity). Interacts (via C-terminus) with KCNB1 (via C-terminus); the interaction increases in a calcium-dependent manner and induces a pore-independent enhancement of exocytosis in neuroendocrine cells, cH2O2affin cells, pancreatic beta cells and from the soma of dorsal root ganglia (DRG) neurons (By similarity). Interacts with SYTL4 (By similarity). Interacts with STXBP6 (By similarity). Interacts with PLCL1 (via C2 domain) (By similarity). Interacts witH2O2OF (By similarity). Interacts with LGI3 (By similarity). Interacts (via the H3 domain) with SLC6A4 (via the N-terminus); this interaction regulates SLC4A6 channel conductance in thalamocortical neurons (By similarity). Interacts with SYT6 and SYT8; the interaction is Ca(2+)-dependent (By similarity). Interacts with VAMP8 (PubMed:12130530). Interacts with SNAP23 (PubMed:12130530). Interacts with VAPA and SYBU (PubMed:15459722). Interacts with PRRT2 (By similarity). Interacts with SEPT8 (By similarity). Interacts with STXBP5L (By similarity). Interacts with synaptotagmin-1/SYT1 (By similarity). Interacts with SEPTIN5; in the cerebellar cortex (By similarity). Interacts with SEPTIN4; in the striatum (By similarity)

RRID

AB_3104368

Database

Entrez Gene: 6804 Human ; 20907 Mouse ; 116470 Rat

SwissProt: Q16623 Human ; O35526 Mouse ; P32851 Rat

OMIM: 186590 Human

Research Field

Neuroscience

Synonyms

STX1A; STX1; Syntaxin-1A; Neuron-specific antigen HPC-1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Syntaxin 1A Antibody (YA2001) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Syntaxin 1A Antibody (YA2001)

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Syntaxin 1A Antibody (YA2001) Related Classifications

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