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MAGEA4 Antibody (YA4022)

(Synonyms: CT1.4; MAGE4; MAGE4A; MAGE4B; MAGE-41; MAGE-X2)
Customer Review

Based on 1 Customer Validation

MAGEA4 Antibody (YA4022) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAGEA4.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MAGEA4 Antibody (YA4022)
  • Experimental Validation Results for MAGEA4 Antibody (YA4022)
  • MAGEA4 Antibody (YA4022) Product Image
  • Experimental Validation Results for MAGEA4 Antibody (YA4022)
  • Experimental Validation Results for MAGEA4 Antibody (YA4022)
  • MAGEA4 Antibody (YA4022) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

MAGEA4 Antibody (YA4022) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAGEA4.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

Purified recombinant fragment of human MAGEA4 (AA: 1-225) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718883

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MAGEA4 Antibody (YA4022)
    Western blot analysis of extracts from Rat liver(lane 1(40μg) ) and Mouse liver(lane 2(40μg) ) using MAGEA4 antibody(HY-P84325). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10
  • Experimental Validation Results for MAGEA4 Antibody (YA4022)
    Western blot analysis of extracts from Rat liver(lane 1(40μg) ) and Mouse liver(lane 2(40μg) ) using MAGEA4 antibody(32234P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:17924661, PubMed:7900999). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed:24204001)

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P35680 has 4 isomers: P35680-1: 61324 Da (predicted); P35680-2: 58379 Da (predicted); P35680-3: 44764 Da (predicted); P35680-4: 46907 Da (predicted).

  • Subunit

    Binds DNA as a dimer. Can form homodimer or heterodimer with HNF1-alpha. Interacts (via HNF-p1 domain) with PCBD1; the interaction increases its transactivation activity (PubMed:24204001)

  • SwissProt ID

    P43358 

  • Gene ID
    4103 [NCBI]
  • Synonyms

    CT1.4; MAGE4; MAGE4A; MAGE4B; MAGE-41; MAGE-X2

MAGEA4 Antibody (YA4022) Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MAGEA4 Antibody (YA4022) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from Rat liver(lane 1(40μg) ) and Mouse liver(lane 2(40μg) ) using MAGEA4 antibody(HY-P84325). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10

  • 1/16

    MAGEA4 Antibody (YA4022) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from Rat liver(lane 1(40μg) ) and Mouse liver(lane 2(40μg) ) using MAGEA4 antibody(32234P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • MAGEA4 Antibody (YA4022) Product Image
    1/16
  • Experimental Validation Results for MAGEA4 Antibody (YA4022)
  • Experimental Validation Results for MAGEA4 Antibody (YA4022)
  • MAGEA4 Antibody (YA4022) Product Image
  • MAGEA4 Antibody (YA4022) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat liver(lane 1(40μg) ) and Mouse liver(lane 2(40μg) ) using MAGEA4 antibody(HY-P84325). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10

  • MAGEA4 Antibody (YA4022) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat liver(lane 1(40μg) ) and Mouse liver(lane 2(40μg) ) using MAGEA4 antibody(32234P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • MAGEA4 Antibody (YA4022) Product Image WB
  • MAGEA4 Antibody (YA4022) Product Image WB