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AER61 Antibody (YA2015)

(Synonyms: Extracellular O-linked N-acetylglucosamine transferase)
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Based on 1 Customer Validation

AER61 Antibody (YA2015) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AER61.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for AER61 Antibody (YA2015)
  • AER61 Antibody (YA2015) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

AER61 Antibody (YA2015) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AER61.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human AER61

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104382

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AER61 Antibody (YA2015)
    Western blot analysis was performed on extracts from U-87MG (lane 1, 20 μg), HeLa (lane 2, 20 μg), Caco-2 (lane 3, 20 μg), C6 (lane 4, 20 μg), 293 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using AER61 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    AER61 catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains

  • Subcellular Localization

    Endoplasmic reticulum lumen

  • Isoforms & Post-Translational Modification

    Q5NDL2 has 3 isomers: Q5NDL2-1: 62011 Da (predicted); Q5NDL2-2: 24403 Da (predicted); Q5NDL2-3: 52297 Da (predicted).

  • SwissProt ID

    Q5NDL2

  • Gene ID
    285203 [NCBI]
  • Synonyms

    Extracellular O-linked N-acetylglucosamine transferase

  • Research Field

    Cell Biology

AER61 Antibody (YA2015) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from U-87MG (lane 1, 20 μg), HeLa (lane 2, 20 μg), Caco-2 (lane 3, 20 μg), C6 (lane 4, 20 μg), 293 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using AER61 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • AER61 Antibody (YA2015) Product Image
    1/16
  • Experimental Validation Results for AER61 Antibody (YA2015)
  • AER61 Antibody (YA2015) Product Image
  • Experimental Validation Results for AER61 Antibody (YA2015)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from U-87MG (lane 1, 20 μg), HeLa (lane 2, 20 μg), Caco-2 (lane 3, 20 μg), C6 (lane 4, 20 μg), 293 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using AER61 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for AER61 Antibody (YA2015)
  • AER61 Antibody (YA2015) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from U-87MG (lane 1, 20 μg), HeLa (lane 2, 20 μg), Caco-2 (lane 3, 20 μg), C6 (lane 4, 20 μg), 293 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using AER61 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • AER61 Antibody (YA2015) Product Image WB