1. Antibodies
  2. Primary Antibodies
  3. Monoclonal Antibodies Tag Antibodies
  4. VSV G tag Antibody (YA875)

VSV G tag Antibody (YA875)

Art. -Nr.: HY-P81031
COA User Guide for Antibodies Technical Support

VSV G tag Antibody (YA875) is an unconjugated, anti-VSV G tag (YA875) IgG1 monoclonal antibody.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

  • Verification Image

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  • Beschreibung

Beschreibung

VSV G tag Antibody (YA875) is an unconjugated, anti-VSV G tag (YA875) IgG1 monoclonal antibody.

Host

Mouse

Clonality

Monoclonal

Molekulargewicht
Predicted band size: 50 kDa;
Observed band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Species independent
Immunogen

Synthetic Peptide of VSV-G-Tag

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:10000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-1:500
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Reinheit Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG1  
Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis was performed on extracts from Recombinant VSV G-Tag protein-10ng (lane 1, 10 ng), Recombinant VSV G-Tag protein-5ng (lane 2, 5 ng), Recombinant VSV G-Tag protein-2ng (lane 3, 2 ng), Recombinant VSV G-Tag protein-1ng (lane 4, 1 ng), Recombinant VSV G-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant VSV G-Tag protein-0.1ng (lane 6, 0.1 ng) using VSV G tag Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function:VSV-G Peptide is a 11 amino acid peptide derived from the Vesicular Stomatitis viral glycoprotein.
Research Field

Tags & Cell Markers

Dokumentation

VSV G tag Antibody (YA875) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Produktname:
VSV G tag Antibody (YA875)
Art. -Nr.:
HY-P81031
Menge:
MCE Japan Authorized Agent: