Hexokinase I Antibody (YA739)(PBS only)

Cat. No.: HY-P80702A: Data Sheet User Guide for Antibodies
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Hexokinase I Antibody (YA739) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Hexokinase I.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Hexokinase I Antibody (YA739) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Hexokinase I.

Host

Mouse

Molecular Weight

Predicted band size: 102 kDa;

Observed band size: 102 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P19367

Gene ID

3098 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Hexokinase 1.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331)

Subcellular Localization：Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasm, cytosol

Expression：
Tissue_specificity:Isotype 2: Erythrocyte specific (Reference 6) . Isotype 3: Testis specific (PubMed: 10978502) . Isotype 4: Testis specific (PubMed: 10978502) .

Isoforms & Post-Translational Modification：P19367 has 4 isomers: P19367-1: 102486 Da (predicted); P19367-2: 102201 Da (predicted); P19367-3: 102738 Da (predicted); P19367-4: 101085 Da (predicted).

Subunit：Monomer (PubMed:10686099). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with VDAC1 (PubMed:22304920). The HK1-VDAC1 complex interacts with ATF2 (PubMed:22304920). Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity). Interacts with SMAD5 (PubMed:28675158)

Research Field

Cardiovascular

Synonyms

HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

Documentation

User Guide for Antibodies (1077 KB)