CD215 Antibody (YA4229)

Cat. No.: HY-P84532: Data Sheet COA
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CD215 Antibody (YA4229) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD215.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

CD215 Antibody (YA4229) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD215.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 28.2 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13261

Gene ID

3601 [NCBI]

Immunogen

Purified recombinant fragment of human CD215 (AA: extra 31-205) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Hela (lane4(20μg) and Ramos (lane5(20μg) using CD215 Antibody (HY-P84532). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：High-affinity receptor for interleukin-15 (PubMed:8530383). Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (By similarity). In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (PubMed:15123770). In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (PubMed:15123770). Expression of different isoforms may alter or interfere with signal transduction (PubMed:10480910); Does not bind IL15; Does not bind IL15; Does not bind IL15; Does not bind IL15

Subcellular Localization：Membrane; Single-pass type I membrane protein; Nucleus membrane; Single-pass type I membrane protein; Cell surface; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Membrane; Single-pass type I membrane protein; Secreted, extracellular space

Expression：
Tissue_specificity:Expression in neutrophils (protein level) (PubMed:15123770) . Expression in the fetal brain, with higher expression in the hippocampus and cerebellum than in the cortex and thalamus (PubMed:12114302) . Soluble sIL-15RA levels are higher in all brain structures than in the membrane-bound type (PubMed:12114302) . Subtypes 1, 3, 4, 5, 6, 7, 8, and 9: widely expressed (PubMed:10480910, PubMed:8530383) .

Subunit：The interleukin-15 receptor IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL15RA also self-associates (PubMed:17643103). Interacts with SYK (PubMed:15123770)

RRID

AB_3718927

Synonyms

IL15RA

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)