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CD215 Antibody (YA4229)

(Synonyms: IL15RA)
Customer Review

Based on 1 Customer Validation

CD215 Antibody (YA4229) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD215.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD215 Antibody (YA4229)
  • CD215 Antibody (YA4229) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:10000

Product Details

Description

CD215 Antibody (YA4229) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD215.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28.2 kDa
Immunogen

Purified recombinant fragment of human CD215 (AA: extra 31-205) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718927

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD215 Antibody (YA4229)
    Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Hela (lane4(20μg) and Ramos (lane5(20μg) using CD215 Antibody (HY-P84532). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CD215 is a High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. In neutrophils, binds and activates kinase SYK in response to IL15 stimulation. In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner. Expression of different isoforms may alter or interfere with signal transduction; Does not bind IL15; Does not bind IL15; Does not bind IL15; Does not bind IL15[1][2][3].

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein; Nucleus membrane; Single-pass type I membrane protein; Cell surface; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Membrane; Single-pass type I membrane protein; Secreted, extracellular space

  • Expression


    Tissue_specificity:Expression in neutrophils (protein level) (PubMed:15123770) . Expression in the fetal brain, with higher expression in the hippocampus and cerebellum than in the cortex and thalamus (PubMed:12114302) . Soluble sIL-15RA levels are higher in all brain structures than in the membrane-bound type (PubMed:12114302) . Subtypes 1, 3, 4, 5, 6, 7, 8, and 9: widely expressed (PubMed:10480910, PubMed:8530383) .

  • Subunit

    The interleukin-15 receptor IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL15RA also self-associates (PubMed:17643103). Interacts with SYK (PubMed:15123770)

  • SwissProt ID

    Q13261 

  • Gene ID
    3601 [NCBI]
  • Synonyms

    IL15RA

CD215 Antibody (YA4229) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Hela (lane4(20μg) and Ramos (lane5(20μg) using CD215 Antibody (HY-P84532). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • CD215 Antibody (YA4229) Product Image
    1/16
  • Experimental Validation Results for CD215 Antibody (YA4229)
  • CD215 Antibody (YA4229) Product Image
  • Experimental Validation Results for CD215 Antibody (YA4229)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Hela (lane4(20μg) and Ramos (lane5(20μg) using CD215 Antibody (HY-P84532). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD215 Antibody (YA4229)
  • CD215 Antibody (YA4229) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Hela (lane4(20μg) and Ramos (lane5(20μg) using CD215 Antibody (HY-P84532). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • CD215 Antibody (YA4229) Product Image WB