Cytokeratin 2e Antibody (YA3977)

Cat. No.: HY-P84280: Ficha de datos COA
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Cytokeratin 2e Antibody (YA3977) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Cytokeratin 2e.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

Cytokeratin 2e Antibody (YA3977) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Cytokeratin 2e.

Host

Mouse

Clonality

Monoclonal Antibody

Peso molecular

Predicted band size: 65 kDa;

Observed band size: 65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P35908

Gene ID

3849 [NCBI]

Immunogen

Purified recombinant fragment of human KRT2 (AA: 177-490) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A431(lane 2(20μg) , Jurkat(lane 3(20μg) ,HepG2(lane 4(20μg)and HeLa(lane 5(20μg) using Cytokeratin 2e Antibody（HY-P84280）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

Background

Function：Probably contributes to terminal cornification (PubMed:1380918). Associated with keratinocyte activation, proliferation and keratinization (PubMed:12598329). Required for maintenance of corneocytes and keratin filaments in suprabasal keratinocytes in the epidermis of the ear, potentially via moderation of expression and localization of keratins and their partner proteins (By similarity). Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:It is expressed in the upper spinous and granular layers of normal adult epidermal tissue in most body parts, including the thighs, nipples, soles of the feet, penile shaft, and armpits. It is not expressed in the foreskin, squamous metaplasia, or carcinoma. In hypertrophic scars and keloids, expression begins in the deepest upper layers. Expression is weak in normal gingiva and tongue, but is enhanced in benign keratosis of the lingual mucosa and mild to moderate oral dysplasia with orthokeratosis.

Induction:Induced in keratinocytes by all-trans retinoic acid (ATRA) , via increase in mRNA stability

Subunit：Heterotetramer of two type I and two type II keratins. Associates with KRT10

Synonyms

K2e; KRTE; CK-2e; KRT2A; KRT2E

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)