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  5. Phospho-JunD (Ser255) Antibody (YA3087)

Phospho-JunD (Ser255) Antibody (YA3087)

Art. -Nr.: HY-P83342
User Guide for Antibodies Technical Support

Phospho-JunD (Ser255) Antibody (YA3087) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-JunD (Ser255).

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Beschreibung

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Beschreibung

Phospho-JunD (Ser255) Antibody (YA3087) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-JunD (Ser255).
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molekulargewicht
Predicted band size: 35 kDa;
Observed band size: 38,42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human JunD GE-pS-PP.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:2000
IP
IP: Immunoprecipitation
1:50
Sensitivity Endogenous Reinheit Affinity Chromatography
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Background
Function：Transcription factor binding AP-1 sites (PubMed:9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed:28981703, PubMed:9989505)
Subcellular Localization：Nucleus
Subunit：Heterodimer; binds DNA as a heterodimer (PubMed:28981703). Component of an AP-1 transcription factor complex composed of JUN-FOS heterodimers (By similarity). As part of the AP-1 transcription factor complex, forms heterodimers with FOS proteins, thereby binding to the AP-1 consensus sequence and stimulating transcription (By similarity). Forms heterodimers with FOSB; thereby binding to the AP-1 consensus sequence (PubMed:28981703). Interacts (via MBM motif) with MEN1; this interaction represses transcriptional activation (PubMed:22327296, PubMed:9989505). Interacts with MAPK10; this interaction is inhibited in the presence of MEN1 (PubMed:22327296)
Database

Entrez Gene: 3727 Human ; 16478 Mouse ; 24518 Rat

SwissProt: P17535 Human ; P15066 Mouse ; P52909 Rat

OMIM: 165162 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
Activator protein 1; AP 1; Jun D; jun D proto oncogene; Jund; JunD FL isoform; Transcription factor jun D
Dokumentation
User Guide for Antibodies (1077 KB)
Verweise

Phospho-JunD (Ser255) Antibody (YA3087) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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