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MCAK Antibody (YA2414)

(Synonyms: KIF2C; KNSL6; Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK)
Customer Review

Based on 1 Customer Validation

MCAK Antibody (YA2414) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCAK.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for MCAK Antibody (YA2414)
  • Experimental Validation Results for MCAK Antibody (YA2414)
  • Experimental Validation Results for MCAK Antibody (YA2414)
  • MCAK Antibody (YA2414) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:50-1:200 1:50-1:100

Product Details

Description

MCAK Antibody (YA2414) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCAK.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 81 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 81 kDa
Species Reactivity Database

Entrez Gene: 11004 Human

SwissProt: Q99661 Human

Immunogen

A synthesized peptide derived from human MCAK aa61-86.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MCAK Antibody (YA2414)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), K562 (lane 3(20μg), Jurkat (lane 4(20μg), HEK293T (lane 5(20μg) using MCAK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MCAK Antibody (YA2414)
    Immunocytochemistry analysis of Hela cells labeling MCAK Antibody with MCAK Antibody (YA2414) (HY-P82669) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCAK Antibody (YA2414) ((HY-P82669) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MCAK Antibody (YA2414)
    Immunocytochemistry analysis of MCF-7 cells labeling MCAK Antibody with MCAK Antibody (YA2414) (HY-P82669) at 1/50dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCAK Antibody (YA2414) ((HY-P82669) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    MCAK is an In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment[1][2][3].

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Nucleus; Chromosome, centromere; Chromosome, centromere, kinetochore

  • Expression


    Tissue_specificity:Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific

  • Isoforms & Post-Translational Modification

    Q99661 has 2 isomers: Q99661-1: 81313 Da (predicted); Q99661-2: 75561 Da (predicted).
    Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity;Ubiquitinated

  • Subunit

    Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding

  • SwissProt ID

    Q99661

  • Gene ID
    11004 [NCBI]
  • Synonyms

    KIF2C; KNSL6; Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK

  • Research Field

    Signal Transduction

MCAK Antibody (YA2414) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), K562 (lane 3(20μg), Jurkat (lane 4(20μg), HEK293T (lane 5(20μg) using MCAK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MCAK Antibody with MCAK Antibody (YA2414) (HY-P82669) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCAK Antibody (YA2414) ((HY-P82669) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling MCAK Antibody with MCAK Antibody (YA2414) (HY-P82669) at 1/50dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCAK Antibody (YA2414) ((HY-P82669) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MCAK Antibody (YA2414) Product Image
    1/16
  • Experimental Validation Results for MCAK Antibody (YA2414)
  • Experimental Validation Results for MCAK Antibody (YA2414)
  • Experimental Validation Results for MCAK Antibody (YA2414)
  • MCAK Antibody (YA2414) Product Image
  • Experimental Validation Results for MCAK Antibody (YA2414)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), K562 (lane 3(20μg), Jurkat (lane 4(20μg), HEK293T (lane 5(20μg) using MCAK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MCAK Antibody (YA2414)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MCAK Antibody with MCAK Antibody (YA2414) (HY-P82669) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCAK Antibody (YA2414) ((HY-P82669) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MCAK Antibody (YA2414)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling MCAK Antibody with MCAK Antibody (YA2414) (HY-P82669) at 1/50dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCAK Antibody (YA2414) ((HY-P82669) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MCAK Antibody (YA2414)
  • Experimental Validation Results for MCAK Antibody (YA2414)
  • Experimental Validation Results for MCAK Antibody (YA2414)
  • MCAK Antibody (YA2414) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), K562 (lane 3(20μg), Jurkat (lane 4(20μg), HEK293T (lane 5(20μg) using MCAK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MCAK Antibody (YA2414) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling MCAK Antibody with MCAK Antibody (YA2414) (HY-P82669) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCAK Antibody (YA2414) ((HY-P82669) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MCAK Antibody (YA2414) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling MCAK Antibody with MCAK Antibody (YA2414) (HY-P82669) at 1/50dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCAK Antibody (YA2414) ((HY-P82669) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MCAK Antibody (YA2414) Product Image WB
  • MCAK Antibody (YA2414) Product Image ICC
  • MCAK Antibody (YA2414) Product Image ICC