KPNA2 Antibody (YA1415)

Cat. No.: HY-P81670: Data Sheet COA
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KPNA2 Antibody (YA1415) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KPNA2.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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KPNA2 Antibody (YA1415) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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KPNA2 Antibody (YA1415) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KPNA2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 58 kDa;

Observed band size: 58 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P52292

Gene ID

3838 [NCBI]

Immunogen

A synthesized peptide derived from human KPNA2 aa50-100/529.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using KPNA2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed:28991411, PubMed:32130408, PubMed:7604027, PubMed:7754385). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed:28991411, PubMed:32130408, PubMed:7604027, PubMed:7754385). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:7604027, PubMed:7754385). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with KPNA1 and Transportin-1/TNPO1 (PubMed:35446349)

Subcellular Localization：Cytoplasm; Nucleus; Endoplasmic reticulum membrane; Golgi apparatus membrane

Expression：
Tissue_specificity:general expression

Subunit：Heterodimer; with KPNB1 (PubMed:10353244, PubMed:17596301). Interacts with ANP32E (By similarity). Component of a complex containing CSE1L, RAN and KPNA2. Interacts directly with CSE1L. Interacts with PLAG1. Interacts with APEX1 (via N-terminus). Interacts with FRG1 (via N-terminus). Interacts with ARL4A, CTNNBL1 and NBN. Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers). Interacts with BAG6 (PubMed:29042515). Interacts with AIFM2; this interaction likely mediates the translocation of AIFM2 into the nucleus upon oxidative stress. Interacts with RSL1D1 (PubMed:36647821). Interacts with BAP1 (via C-terminus); the interaction contributes to BAP1 nuclear localization (PubMed:35446349). Interacts with PLSCR4; this interaction mediates the nucleus import of PLSCR4 (PubMed:21690087)

RRID

AB_3103779

Database

Entrez Gene: 3838 Human ; 16647 Mouse ; 85245 Rat

SwissProt: P52292 Human ; P52293 Mouse ;

OMIM: 600685 Human

Research Field

Immunology

Synonyms

KPNA2; RCH1; SRP1; Importin subunit alpha-2; Karyopherin subunit alpha-2; RAG cohort protein 1; SRP1-alpha

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

KPNA2 Antibody (YA1415) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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KPNA2 Antibody (YA1415)

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Background

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KPNA2 Antibody (YA1415) Related Classifications

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