Phospho-NLRC4 (S533) Antibody (YA6970)

Cat. No.: HY-P87287: Data Sheet User Guide for Antibodies
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Phospho-NLRC4 (S533) Antibody (YA6970) is a Rat-derived and non-conjugated IgG2b monoclonal antibody, targeting to Phospho-NLRC4 (S533).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Phospho-NLRC4 (S533) Antibody (YA6970) is a Rat-derived and non-conjugated IgG2b monoclonal antibody, targeting to Phospho-NLRC4 (S533).

Host

Rat

Clonality

Monoclonal

Molecular Weight

Predicted band size: 117 kDa;

Observed band size: 117 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q3UP24

Immunogen

Synthesized peptide of mouse phospho-NLRC4(Ser-533) (AA: 525-538) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. It senses pathogenic proteins of the type III secretion system (T3SS) and type IV secretion system (T4SS) such as flagellin and PrgJ-like rod proteins via the Naip proteins (Naip1, Naip2 or Naip5): specific Naip proteins recognize and bind pathogenic proteins, driving assembly and activation of the NLRC4 inflammasome. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri and fungal pathogen C.albicans. In intestine, the NLRC4 inflammasome is able to discriminate between commensal and pathogenic bacteria and specifically drives production of interleukin-1 beta (IL1B) in response to infection by Salmonella or P.aeruginosa. In case of L.pneumophila infection the inflammasome acts by activating caspase-7

Subcellular Localization：Cytoplasm, cytosol,Inflammasome

Expression：
Tissue_Specificity: Expressed by intestinal mononuclear phagocytes

Subunit：Homooligomer; homooligomerizes following activation of Naip proteins by pathogenic proteins such as S

Synonyms

and NACHT-containing protein antibody; CARD 12 antibody; CARD antibody; CARD LRR and NACHT containing protein 1 antibody; CARD LRR and NACHT containing protein antibody; CARD12 antibody; Caspase recruitment domain containing protein 12 antibody; Caspase recruitment domain family member 12 antibody; Caspase recruitment domain-containing protein 12 antibody; CLAN 1 antibody

Documentation

User Guide for Antibodies (1077 KB)