Copy Link Print Information

NGB Antibody (YA4512)

Customer Review

Based on 1 Customer Validation

NGB Antibody (YA4512) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NGB.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NGB Antibody (YA4512)
  • NGB Antibody (YA4512) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

NGB Antibody (YA4512) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NGB.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Immunogen

Purified recombinant fragment of human NGB(AA: full 1-151) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NGB Antibody (YA4512)
    Western blot analysis of extracts from A549(lane 2(20μg) , Hela(lane 3(20μg) ,U251(lane 4(20μg)and HepG2( lane 5(20μg) using NGB Antibody (HY-P84815). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    NGB is a Monomeric globin with a bis-histidyl six-coordinate heme-iron atom through which it can bind dioxygen, carbon monoxide and nitric oxide. Could help transport oxygen and increase its availability to the metabolically active neuronal tissues, though its low quantity in tissues as well as its high affinity for dioxygen, which may limit its oxygen-releasing ability, argue against it. The ferrous/deoxygenated form exhibits a nitrite reductase activity and it could produce nitric oxide which in turn inhibits cellular respiration in response to hypoxia. In its ferrous/deoxygenated state, it may also exhibit GDI (Guanine nucleotide Dissociation Inhibitor) activity toward heterotrimeric G-alpha proteins, thereby regulating signal transduction to facilitate neuroprotective responses in the wake of hypoxia and associated oxidative stress[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasm, cytosol; Mitochondrion matrix

  • Expression


    Tissue_specificity:Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus

  • Subunit

    Monomer (PubMed:11029004). Homodimer and homotetramer; disulfide-linked.

  • SwissProt ID

    Q9NPG2

  • Gene ID
    58157 [NCBI]

NGB Antibody (YA4512) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20μg) , Hela(lane 3(20μg) ,U251(lane 4(20μg)and HepG2( lane 5(20μg) using NGB Antibody (HY-P84815). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • NGB Antibody (YA4512) Product Image
    1/16
  • Experimental Validation Results for NGB Antibody (YA4512)
  • NGB Antibody (YA4512) Product Image
  • Experimental Validation Results for NGB Antibody (YA4512)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20μg) , Hela(lane 3(20μg) ,U251(lane 4(20μg)and HepG2( lane 5(20μg) using NGB Antibody (HY-P84815). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NGB Antibody (YA4512)
  • NGB Antibody (YA4512) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20μg) , Hela(lane 3(20μg) ,U251(lane 4(20μg)and HepG2( lane 5(20μg) using NGB Antibody (HY-P84815). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • NGB Antibody (YA4512) Product Image WB