Glucose 6 Phosphate Dehydrogenase Antibody (YA411)(PBS only)

Cat. No.: HY-P80688A: Fiche technique User Guide for Antibodies
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Glucose 6 Phosphate Dehydrogenase Antibody (YA411) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

* Veuillez sélectionner la quantité avant d'ajouter des articles.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

Glucose 6 Phosphate Dehydrogenase Antibody (YA411) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

Host

Rabbit

Masse moléculaire

Predicted band size: 59 kDa;

Observed band size: 59 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P11413

Gene ID

2539 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Glucose 6 Phosphate Dehydrogenase.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Pureté	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis

Subcellular Localization：Cytoplasm, cytosol; Membrane; Peripheral membrane protein

Expression：
Tissue_specificity:The long subtype is found in lymphoblasts, granulocytes, and sperm.

Isoforms & Post-Translational Modification：P11413 has 3 isomers: P11413-1: 59257 Da (predicted); P11413-2: 63827 Da (predicted); P11413-3: 62468 Da (predicted).
Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity

Subunit：Homotetramer; dimer of dimers (PubMed:10745013, PubMed:15858258, PubMed:24769394, PubMed:38066190). Interacts with SIRT2; the interaction is enhanced by H(2)O(2) treatment (PubMed:24769394). Forms a ternary complex with ALDOB and TP53; this interaction is direct. ALDOB stabilizes the complex inhibiting G6PD activity and keeping oxidative pentose phosphate metabolism in check

Research Field

Signal Transduction

Synonyms

G6PD; Glucose-6-phosphate 1-dehydrogenase; G6PD

Documentation

User Guide for Antibodies (1077 KB)