ERR alpha Antibody (YA3761)

Cat. No.: HY-P84064: Fiche technique COA
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ERR alpha Antibody (YA3761) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ERR alpha.

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Size	Stock	Quantité
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description

Description

ERR alpha Antibody (YA3761) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ERR alpha.

Host

Mouse

Clonality

Monoclonal Antibody

Masse moléculaire

Predicted band size: 46 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P11474

Gene ID

2101 [NCBI]

Immunogen

Purified recombinant fragment of human ESRRA (AA: 198-376) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureté	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MCF-7 (lane 2(20μg), HeLa (lane 3(20μg), HT-29 (lane 4(20μg) and SiHa (lane 5(20μg) using ERR alpha Antibody (HY-P84064) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Induction:Induced by PGC1alpha in a number of specific cell types including heart, kidney and muscle

Subunit：Binds DNA as a monomer or a homodimer. Interacts (via the AF2 domain) with coactivator PPARGC1A (via the L3 motif); the interaction greatly enhances transcriptional activity of genes involved in energy metabolism. Interacts with PIAS4; the interaction enhances sumoylation. Interacts with MAPK15; promotes re-localization of ESRRA to the cytoplasm through a XPO1-dependent mechanism then inhibits ESRRA transcriptional activity

RRID

AB_3718825

Synonyms

ERR1; ERRa; ESRL1; NR3B1; ERRalpha

Documentation

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Fiche technique (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)