EphA5 Antibody (YA4833)

Cat. No.: HY-P85141: Data Sheet User Guide for Antibodies
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EphA5 Antibody (YA4833) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to EphA5.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

EphA5 Antibody (YA4833) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to EphA5.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 115 kDa;

Species Reactivity

Human

SwissProt ID

P54756

Gene ID

2044 [NCBI]

Immunogen

Purified recombinant fragment of EphA5 (aa620-774) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell projection, axon; Cell projection, dendrite

Expression：
Tissue_specificity:Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord

Isoforms & Post-Translational Modification：P54756 has 3 isomers: P54756-1: 114803 Da (predicted); P54756-2: 112624 Da (predicted); P54756-3: 107699 Da (predicted).
Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5. Dephosphorylation upon stimulation by glucose, inhibits EPHA5 forward signaling and results in insulin secretion (By similarity)

Subunit：Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer.

Synonyms

EPHA5; BSK; EHK1; HEK7; TYRO4; Ephrin type-A receptor 5; Brain-specific kinase; EPH homology kinase 1; EHK-1; EPH-like kinase 7; EK7; hEK7

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/l02-cell.html [Content Brief]