SAFB1 Antibody (YA5230)

Cat. No.: HY-P85538: Ficha de datos User Guide for Antibodies
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SAFB1 Antibody (YA5230) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SAFB1.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

SAFB1 Antibody (YA5230) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SAFB1.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Observed band size: 130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q15424

Gene ID

6294 [NCBI]

Immunogen

Purified recombinant human SAFB1 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200

Pureza	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (PubMed:9671816). Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription (PubMed:12660241). Thereby acts as a negative regulator of cell proliferation (PubMed:12660241). When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It is widely distributed. It is highly expressed in the central nervous system and lowly expressed in the liver. It is also expressed in various breast cancer cell lines.

Isoforms & Post-Translational Modification：Q15424 has 4 isomers: Q15424-1: 102642 Da (predicted); Q15424-2: 95181 Da (predicted); Q15424-3: 102855 Da (predicted); Q15424-4: 102768 Da (predicted).
Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity

Subunit：Monomer and homodimer (PubMed:12660241). Forms heterodimers with SAFB2 (PubMed:12660241). Interacts with KHDRBS3 (By similarity). Interacts with CLK2 (By similarity). Interacts with POLR2A, SRSF1/ASF, SRSF9/SRp30c and SFSF10/TRA2B (PubMed:9671816). Interacts with isoform 1 and isoform 2 of SRPK1 and inhibits its activity (PubMed:19674106). Interacts with RBMX (By similarity). Interacts with FUS (PubMed:27731383). Interacts with ZBED4 (PubMed:22693546)

Synonyms

DKFZp779C1727; glutathione S transferase fusion protein; HAP; HET; HSP27 ERE TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAF B; SAF-B1; SAFB 1; SAFB; SAFB1; SAFB1_HUMAN; scaffold attachment factor B1.

Documentación

User Guide for Antibodies (1077 KB)