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NEDD8 Antibody (YA273)

(Synonyms: FLJ43224 antibody; MGC104393 antibody; MGC125896 antibody; MGC125897 antibody; NED8 antibody; NEDD 8 antibody; NEDD-8 antibody; Nedd8 antibody; NEDD8_HUMAN antibody; Neddylin antibody; Neural precursor cell expressed developmentally down regulated 8 antibody; Neural precursor cell expressed developmentally down regulated gene 8 antibody; Neural precursor cell expressed developmentally down-regulated protein 8 antibody; Rub1 antibody; Ubiquitin like protein Nedd 8 antibody; Ubiquitin like protein Nedd8 antibody; Ubiquitin-like protein Nedd8 antibody; )
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Based on 1 publication(s) in Google Scholar

NEDD8 Antibody (YA273) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEDD8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • NEDD8 Antibody (YA273) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100 1:50

Product Details

Description

NEDD8 Antibody (YA273) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEDD8.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 9-90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 9 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human NEDD8.AA range:12-57.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102372

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NEDD8 Antibody (YA273)
    Western blot analysis of extracts from Hela (lane 2(20μg) , HepG2(lane 3(20μg) , and K562 (lane 4(20μg)using NEDD8 (HY-P80240) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80240, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NEDD8 Antibody (YA273)
    Immunocytochemistry analysis of Hela cells labeling NEDD8 with Cleaved NEDD8 Antibody (HY-P80240) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEDD8 Antibody (HY-P80240) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NEDD8 Antibody (YA273)
    Immunocytochemistry analysis of HepG2 cells labeling NEDD8 with NEDD8 Antibody (HY-P80240) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEDD8 Antibody (HY-P80240)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NEDD8 Antibody (YA273)
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using NEDD8 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80240, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NEDD8 Antibody (YA273)
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using NEDD8 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80240, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:14690597, PubMed:15242646, PubMed:9694792, PubMed:38605244, PubMed:38316879). Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:20688984, PubMed:9694792, PubMed:38605244, PubMed:38316879). Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity (PubMed:15242646). Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (PubMed:14690597)

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is highly expressed in the heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon, and leukocytes.

  • Subunit

    Interacts with AHR; interaction is direct (PubMed:12215427). Interacts with NUB1; interaction is direct (PubMed:11259415). Interacts with ESR1 (By similarity)

  • SwissProt ID

    Q15843

  • Gene ID
    4738 [NCBI]
  • Synonyms

    FLJ43224 antibody; MGC104393 antibody; MGC125896 antibody; MGC125897 antibody; NED8 antibody; NEDD 8 antibody; NEDD-8 antibody; Nedd8 antibody; NEDD8_HUMAN antibody; Neddylin antibody; Neural precursor cell expressed developmentally down regulated 8 antibody; Neural precursor cell expressed developmentally down regulated gene 8 antibody; Neural precursor cell expressed developmentally down-regulated protein 8 antibody; Rub1 antibody; Ubiquitin like protein Nedd 8 antibody; Ubiquitin like protein Nedd8 antibody; Ubiquitin-like protein Nedd8 antibody;

  • Research Field

    Cell Biology

NEDD8 Antibody (YA273) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , HepG2(lane 3(20μg) , and K562 (lane 4(20μg)using NEDD8 (HY-P80240) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80240, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling NEDD8 with Cleaved NEDD8 Antibody (HY-P80240) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEDD8 Antibody (HY-P80240) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling NEDD8 with NEDD8 Antibody (HY-P80240) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEDD8 Antibody (HY-P80240)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using NEDD8 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80240, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using NEDD8 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80240, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • NEDD8 Antibody (YA273) Product Image
    1/16
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • NEDD8 Antibody (YA273) Product Image
  • Experimental Validation Results for NEDD8 Antibody (YA273)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , HepG2(lane 3(20μg) , and K562 (lane 4(20μg)using NEDD8 (HY-P80240) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80240, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NEDD8 Antibody (YA273)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling NEDD8 with Cleaved NEDD8 Antibody (HY-P80240) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEDD8 Antibody (HY-P80240) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NEDD8 Antibody (YA273)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling NEDD8 with NEDD8 Antibody (HY-P80240) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEDD8 Antibody (HY-P80240)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NEDD8 Antibody (YA273)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using NEDD8 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80240, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NEDD8 Antibody (YA273)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using NEDD8 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80240, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • Experimental Validation Results for NEDD8 Antibody (YA273)
  • NEDD8 Antibody (YA273) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) , HepG2(lane 3(20μg) , and K562 (lane 4(20μg)using NEDD8 (HY-P80240) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80240, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • NEDD8 Antibody (YA273) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling NEDD8 with Cleaved NEDD8 Antibody (HY-P80240) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEDD8 Antibody (HY-P80240) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NEDD8 Antibody (YA273) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling NEDD8 with NEDD8 Antibody (HY-P80240) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEDD8 Antibody (HY-P80240)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NEDD8 Antibody (YA273) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using NEDD8 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80240, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • NEDD8 Antibody (YA273) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using NEDD8 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80240, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • NEDD8 Antibody (YA273) Product Image WB
  • NEDD8 Antibody (YA273) Product Image ICC
  • NEDD8 Antibody (YA273) Product Image ICC
  • NEDD8 Antibody (YA273) Product Image IHC-P
  • NEDD8 Antibody (YA273) Product Image IHC-P