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CREB1 Antibody (YA3823)

(Synonyms: CREB; MGC9284; CREB1)
Customer Review

Based on 1 Customer Validation

CREB1 Antibody (YA3823) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CREB1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • CREB1 Antibody (YA3823) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:10000

Product Details

Description

CREB1 Antibody (YA3823) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CREB1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 35/43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

Purified recombinant fragment of human CREB1 aa 93-294.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718841

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Western blot analysis of extracts from SH-SY5Y(lane 2(20μg), Jurkat(lane 3(20μg), Neuro-2a(lane 4(20μg), A549(lane 5(20μg) using CREB1 Antibody (HY-P84126). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Immunohistochemical analysis of paraffin-embedded human brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Immunohistochemical analysis of paraffin-embedded human breast tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CREB1 is a Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling[1].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P16220 has 3 isomers: P16220-2: 35136 Da (predicted); P16220-1: 36688 Da (predicted); P16220-3: 25445 Da (predicted).
    Stimulated by phosphorylation. Phosphorylation of both Ser-119 and Ser-128 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-119 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). CREBL2 positively regulates phosphorylation at Ser-119 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-119. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-128. Phosphorylation of Ser-128 blocks CREB-mediated transcription even when Ser-119 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-257 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-119 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Phosphorylated by TSSK4 on Ser-119 (PubMed:15964553);Sumoylated with SUMO1. Sumoylation on Lys-290, but not on Lys-271, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization

  • Subunit

    Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators CRTC1/TORC1, CRTC2/TORC2 and CRTC3/TORC3. When phosphorylated on Ser-119, binds CREBBP (By similarity). Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity (By similarity). Interacts (phosphorylated form) with TOX3. Interacts with ARRB1. Binds to HIPK2. Interacts with SGK1. Interacts with TSSK4; this interaction facilitates phosphorylation on Ser-119 (PubMed:15964553). Forms a complex with KMT2A and CREBBP (By similarity) (PubMed:14506290, PubMed:14536081, PubMed:15454081, PubMed:15733869, PubMed:15964553, PubMed:16325578, PubMed:16908542, PubMed:20573984, PubMed:21172805, PubMed:23651431). Interacts with TOX4; CREB1 is required for full induction of TOX4-dependent activity and the interaction is increased by cAMP and inhibited by insulin (By similarity)

  • SwissProt ID

    P16220

  • Gene ID
    1385 [NCBI]
  • Synonyms

    CREB; MGC9284; CREB1

CREB1 Antibody (YA3823) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20μg), Jurkat(lane 3(20μg), Neuro-2a(lane 4(20μg), A549(lane 5(20μg) using CREB1 Antibody (HY-P84126). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image
    1/16
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • CREB1 Antibody (YA3823) Product Image
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20μg), Jurkat(lane 3(20μg), Neuro-2a(lane 4(20μg), A549(lane 5(20μg) using CREB1 Antibody (HY-P84126). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • Experimental Validation Results for CREB1 Antibody (YA3823)
  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SH-SY5Y(lane 2(20μg), Jurkat(lane 3(20μg), Neuro-2a(lane 4(20μg), A549(lane 5(20μg) using CREB1 Antibody (HY-P84126). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CREB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84126, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using CREB1 Antibody (HY-P84126, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CREB1 Antibody (YA3823) Product Image WB
  • CREB1 Antibody (YA3823) Product Image IHC-P
  • CREB1 Antibody (YA3823) Product Image IHC-P
  • CREB1 Antibody (YA3823) Product Image IHC-P
  • CREB1 Antibody (YA3823) Product Image IHC-P
  • CREB1 Antibody (YA3823) Product Image IHC-P
  • CREB1 Antibody (YA3823) Product Image IHC-P
  • CREB1 Antibody (YA3823) Product Image IHC-P
  • CREB1 Antibody (YA3823) Product Image IHC-P
  • CREB1 Antibody (YA3823) Product Image IHC-P