Nur77 Antibody (YA7536)

Cat. No.: HY-P87851: Data Sheet User Guide for Antibodies
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Nur77 Antibody (YA7536) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nur77.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

Nur77 Antibody (YA7536) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nur77.

Host

Rabbit

Clonality

Monoclonal,Recombinant

분자량

Predicted band size: 65 kDa;

Observed band size: 65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P22736

Gene ID

3164 [NCBI]

Immunogen

Synthetic peptide within Human NUR77 aa 10-49 / 598.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2,000-1:5,000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:1,000
FC FC: Flow Cytometry	1:1,000

Sensitivity	Endogenous	Purity	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed:18690216, PubMed:8121493, PubMed:9315652). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (PubMed:18690216). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (PubMed:22983157). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (By similarity). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (PubMed:15466594). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (By similarity). Plays a role in the vascular response to injury (By similarity)|In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7

Subcellular Localization：Nucleus,Cytoplasm, cytosol,Mitochondrion

Expression：
Tissue_Specificity: Fetal muscle and adult liver, brain and thyroid
Induction: Induced by cytosporone B (Csn-B); directly stimulates its own expression (PubMed:18690216). Induced by corticotropin-releasing hormone (CRH) (PubMed:9315652). Induced by growth-stimulating agents (PubMed:1651101)

Isoforms & Post-Translational Modification：P22736 has three isomers: P22736-1: 64463 Da (predicted); P22736-2: 65998 Da (predicted); P22736-3: 33754 Da (predicted).
Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli丨Acetylated by p300/CBP, acetylation increases stability

Subunit：Binds the NGFI-B response element (NBRE) as a monomer (PubMed:18690216)

각종 서류

User Guide for Antibodies (1077 KB)