CD44 Antibody (YA3819)(PBS only)
(Synonyms: IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL)CD44 Antibody (YA3819) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD44.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse, Rat, Monkey
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:200-1:1000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
CD44 Antibody (YA3819) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD44.
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Host Mouse
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Species ReactivityHuman, Mouse, Rat, Monkey
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Observed Molecular WeightObserved band size: 82 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 82 kDa
Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD44 is a hyaluronic acid receptor and cell adhesion molecule that plays a broad role in cell migration, immune cell activation, stem cell maintenance, and tumorigenesis. CD44 serves as both a key marker for memory T cells and a classic surface marker in research on mesenchymal stem cells and cancer stem cells.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Cell projection, microvillus; Secreted
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Expression
Tissue_specificity:Protein levels were detected in fibroblasts and urine (PubMed:25326458, PubMed:36213313, PubMed:37453717) . Protein levels were also detected in the placenta (PubMed:32337544) . Isomer 10 (epithelial isomer) is expressed in epithelial cells and is highly expressed in cancer cells. Expression is suppressed in neuroblastoma cells. -
Isoforms & Post-Translational Modification
CD44 has 19 isomers: P16070-1: 81538 Da (predicted); P16070-2: 3327 Da (predicted); P16070-3: 77983 Da (predicted); P16070-4: 76612 Da (predicted); P16070-5: 80790 Da (predicted); P16070-6: 76705 Da (predicted); P16070-7: 78446 Da (predicted); P16070-8: 74388 Da (predicted); P16070-9: 74196 Da (predicted); P16070-10: 53411 Da (predicted); P16070-11: 46565 Da (predicted); P16070-12: 39416 Da (predicted); P16070-13: 46261 Da (predicted); P16070-14: 43169 Da (predicted); P16070-15: 32075 Da (predicted); P16070-16: 73150 Da (predicted); P16070-17: 75957 Da (predicted); P16070-18: 37278 Da (predicted); P16070-19: 15635 Da (predicted).
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors;N-glycosylated;O-glycosylated; contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638;Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672 -
Subunit
Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with UNC119. Interacts with PDPN (via extracellular domain); this interaction is required for PDPN-mediated directional migration and regulation of lamellipodia extension/stabilization during cell spreading and migration. Interacts with EGFR.
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SwissProt ID
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Synonyms
IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL
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