Phospho-EB3 (Ser162) Antibody (YA9794)
(Synonyms: EB3, EBF3, MAPRE3, RP/EB Family, RP3, APC binding protein, EB 3, EB1 protein family member 3,)Phospho-EB3 (Ser162) Antibody (YA9794) is a Mouse-derived and non-conjugated IgG2b Monoclonal antibody, targeting to Phospho-EB3(Ser162).
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 0.09% sodium azide as a preservative.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-100 |
Product Details
Phospho-EB3 (Ser162) Antibody (YA9794) is a Mouse-derived and non-conjugated IgG2b Monoclonal antibody, targeting to Phospho-EB3(Ser162).
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 32 kDa;
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 32 kDa
Synthetic S162 phosphorylated EB3 peptide conjugated to carrier protein
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 0.09% sodium azide as a preservative.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
EB3 is a Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules[1][2].
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Subcellular Localization
Cytoplasm, cytoskeleton
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Expression
Tissue_Specificity: Predominantly expressed in brain and muscle. -
Isoforms & Post-Translational Modification
Phospho-EB3 has 2 isoforms, Q9UPY8-1: amino acid length is 281, molecular weight is 31982 Da (predicted); Q9UPY8-2: amino acid length is 266, molecular weight is 30380 Da (predicted).
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
EB3, EBF3, MAPRE3, RP/EB Family, RP3, APC binding protein, EB 3, EB1 protein family member 3,
Documentation
References
[1]. Komarova Y, et al. Mammalian end binding proteins control persistent microtubule growth. J Cell Biol. 2009 Mar 9;184(5):691-706. [Content Brief]
[2]. Yang C, et al. EB1 and EB3 regulate microtubule minus end organization and Golgi morphology. J Cell Biol. 2017 Oct 2;216(10):3179-3198. [Content Brief]
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