PAK3 Antibody (YA1367)
(Synonyms: PAK3; OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3)PAK3 Antibody (YA1367) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAK3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:200 | 1:20 |
Product Details
PAK3 Antibody (YA1367) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAK3.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 62 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 62 kDa
Entrez Gene: 5063 Human ; 18481 Mouse ; 29433 Rat
SwissProt: O75914 Human ; Q61036 Mouse ; Q62829 Rat
OMIM: 300558 Human
A synthesized peptide derived from human PAK3 aa1-21.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PAK3 (p21-activated kinase 3) is a Group I serine/threonine kinase that functions downstream of the small GTPases RAC1 and CDC42, linking extracellular signals to cytoskeletal remodeling and neuronal signaling networks[1][2]. Mechanistically, PAK3 regulates dendritic development, dendritic spine morphogenesis, synapse formation, and synaptic plasticity, thereby contributing to the establishment and maintenance of neuronal connectivity[1][2][3]. Through its role in actin-dependent structural remodeling, PAK3 participates in neuroplastic processes that are essential for learning, memory, and higher cognitive functions[1][3]. In disease contexts, genetic alterations of PAK3 are strongly associated with X-linked intellectual disability and broader neurodevelopmental disorders, highlighting the importance of tightly controlled PAK3 signaling during brain development and function[2][4]. Experimental studies further demonstrate that impaired PAK3 activity can disrupt hippocampal plasticity and cognitive performance, supporting its utility as a mechanistic model for investigating neurodevelopmental pathogenesis[5]. Compared with the closely related Group I isoforms PAK1 and PAK2, PAK3 exhibits prominent neuronal expression and has been particularly linked to synapse-associated functions, although all three kinases share substantial sequence homology and overlapping regulatory mechanisms[2]. For experimental applications, PAK3 is commonly studied through genetic perturbation models and by using Group I PAK inhibitors, including pan-PAK compounds such as FRAX597, which enable investigation of PAK-dependent signaling pathways and cytoskeletal regulation in cellular and disease-relevant systems[6].
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Subcellular Localization
Cytoplasm
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Expression
Tissue_specificity:Limited to the nervous system. Highly expressed in postmitotic neurons of the cerebral cortex and hippocampus during development and after birth. -
Isoforms & Post-Translational Modification
O75914 has 4 isomers: O75914-1: 62310 Da (predicted); O75914-2: 60693 Da (predicted); O75914-3: 64530 Da (predicted); O75914-4: 62913 Da (predicted).
Autophosphorylated when activated by CDC42/p21;Neddylated -
Subunit
Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK.
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SwissProt ID
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Synonyms
PAK3; OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3
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Research Field
Neuroscience
Documentation
References
[1]. Kreis P, et al. PAK signalling in neuronal physiology. Cell Signal. 2009 Mar;21(3):384-93. [Content Brief]
[2]. Meng J, et al. Abnormal long-lasting synaptic plasticity and cognition in mice lacking the mental retardation gene Pak3. J Neurosci. 2005 Jul 13;25(28):6641-50. [Content Brief]
[3]. Licciulli S, et al. FRAX597, a small molecule inhibitor of the p21-activated kinases, inhibits tumorigenesis of neurofibromatosis type 2 (NF2)-associated Schwannomas. J Biol Chem. 2013 Oct 4;288(40):29105-14. [Content Brief]
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