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CD64 Antibody (YA3511)

(Synonyms: Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRIa; CD64)
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Based on 1 Customer Validation

CD64 Antibody (YA3511) is a Rabbit-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD64.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD64 Antibody (YA3511)
  • CD64 Antibody (YA3511) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

CD64 Antibody (YA3511) is a Rabbit-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD64.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 43-80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database

Entrez Gene: 2209 Human

SwissProt: P12314 Human

Immunogen

Human FCGR1A

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102784

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD64 Antibody (YA3511)
    Western blot analysis of extracts from THP-1 (lane 2(20μg), THP-1 (lane 3(40μg), using FCGR1A Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    FcγRI (CD64) is a high-affinity receptor for the Fc region of immunoglobulin γ. FcγRI (CD64) is constitutively expressed by monocytes, macrophages, and many myeloid progenitor cells. FcγRI (CD64) plays a role in both innate and adaptive immune responses. FcγRI (CD64) mediates the effector function of IgG on monocytes, thereby triggering antibody-dependent cytotoxicity (ADCC) to kill virus-infected cells.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Monocyte/macrophage specific

  • Isoforms & Post-Translational Modification

    P12314 has 2 isomers: P12314-1: 42632 Da (predicted); P12314-2: 38795 Da (predicted).
    Phosphorylated on serine residues

  • Subunit

    Interacts with IGHG1 (PubMed:11711607). Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN

  • SwissProt ID

    P12314

  • Gene ID
    2209 [NCBI]
  • Synonyms

    Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRIa; CD64

[1]. van Vugt MJ, et al. The FcgammaRIa (CD64) ligand binding chain triggers major histocompatibility complex class II antigen presentation independently of its associated FcR gamma-chain. Blood. 1999 Jul 15;94(2):808-17. [Content Brief]

CD64 Antibody (YA3511) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg), THP-1 (lane 3(40μg), using FCGR1A Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD64 Antibody (YA3511) Product Image
    1/16
  • Experimental Validation Results for CD64 Antibody (YA3511)
  • CD64 Antibody (YA3511) Product Image
  • Experimental Validation Results for CD64 Antibody (YA3511)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg), THP-1 (lane 3(40μg), using FCGR1A Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD64 Antibody (YA3511)
  • CD64 Antibody (YA3511) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from THP-1 (lane 2(20μg), THP-1 (lane 3(40μg), using FCGR1A Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD64 Antibody (YA3511) Product Image WB