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  4. R Cadherin Antibody (YA1844)

R Cadherin Antibody (YA1844)

Cat. No.: HY-P82099
COA User Guide for Antibodies Technical Support

R Cadherin Antibody (YA1844) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to R Cadherin.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

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  • Documentation

Description

R Cadherin Antibody (YA1844) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to R Cadherin.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight
Predicted band size: 100 kDa;
Observed band size: 130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human R Cadherin

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis of extracts from U87-MG(lane 2(20ug) , C6(lane 3(20ug)and Hela(lane 4(20ug) using R Cadherin Antibody (HY-P82099) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function:Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development
Subcellular Localization:Cell membrane; Single-pass type I membrane protein
Expression:
Tissue_specificity:It is primarily expressed in the brain, but also exists in other tissues.
Isoforms & Post-Translational Modification:P55283 has 2 isomers: P55283-1: 100281 Da (predicted); P55283-2: 92388 Da (predicted).
RRID
Database
Research Field

Tags & Cell Markers

Synonyms
cadherin 4; type 1; R-cadherin (retinal); CAD4; RCAD; R-CAD
Documentation

R Cadherin Antibody (YA1844) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
R Cadherin Antibody (YA1844)
Cat. No.:
HY-P82099
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