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R Cadherin Antibody (YA1844)

(Synonyms: cadherin 4; type 1; R-cadherin (retinal); CAD4; RCAD; R-CAD)
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Based on 1 Customer Validation

R Cadherin Antibody (YA1844) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to R Cadherin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for R Cadherin Antibody (YA1844)
  • R Cadherin Antibody (YA1844) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

R Cadherin Antibody (YA1844) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to R Cadherin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 100 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human R Cadherin

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104214

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for R Cadherin Antibody (YA1844)
    Western blot analysis of extracts from U87-MG(lane 2(20ug) , C6(lane 3(20ug)and Hela(lane 4(20ug) using R Cadherin Antibody (HY-P82099) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    R Cadherin is a Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is primarily expressed in the brain, but also exists in other tissues.

  • Isoforms & Post-Translational Modification

    P55283 has 2 isomers: P55283-1: 100281 Da (predicted); P55283-2: 92388 Da (predicted).

  • SwissProt ID

    P55283

  • Gene ID
    1002 [NCBI]
  • Synonyms

    cadherin 4; type 1; R-cadherin (retinal); CAD4; RCAD; R-CAD

  • Research Field

    Tags & Cell Markers

R Cadherin Antibody (YA1844) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U87-MG(lane 2(20ug) , C6(lane 3(20ug)and Hela(lane 4(20ug) using R Cadherin Antibody (HY-P82099) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • R Cadherin Antibody (YA1844) Product Image
    1/16
  • Experimental Validation Results for R Cadherin Antibody (YA1844)
  • R Cadherin Antibody (YA1844) Product Image
  • Experimental Validation Results for R Cadherin Antibody (YA1844)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U87-MG(lane 2(20ug) , C6(lane 3(20ug)and Hela(lane 4(20ug) using R Cadherin Antibody (HY-P82099) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for R Cadherin Antibody (YA1844)
  • R Cadherin Antibody (YA1844) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U87-MG(lane 2(20ug) , C6(lane 3(20ug)and Hela(lane 4(20ug) using R Cadherin Antibody (HY-P82099) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • R Cadherin Antibody (YA1844) Product Image WB