TRIM69 Antibody
(Synonyms: RNF36, HSD-34, HSD34, TRIM69, E3 ubiquitin-protein ligase TRIM69, RFP-like domain-containing protein trimless, RING finger protein 36, RING-type E3 ubiquitin transferase TRIM69, Tripartite motif-containing protein 69)TRIM69 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to TRIM69.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
TRIM69 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to TRIM69.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 32; 34; 39; 57 kDa
Purified recombinant protein of human TRIM69
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TRIM69 is an E3 ubiquitin ligase that plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis indiana virus. Ubiquitinates viral proteins such as dengue virus NS3 thereby limiting infection. In addition, acts as a key mediator of type I interferon induced microtubule stabilization by directly associating to microtubules independently of its E3 ligase activity. Also plays a role in cataract formation together with TP53. Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination. Regulates centrosome dynamics and mitotic progression by ubiquitinating STK3/MST2; leading to its redistribution to the perinuclear cytoskeleton and subsequent phosphorylation by PLK1[1][2][3][4][5][6][7].
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Subcellular Localization
Cytoplasm; Nucleus; Nucleus speckle; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Expression
Induction: By interferon-beta. -
Isoforms & Post-Translational Modification
TRIM69 has 4 isoforms, Q86WT6-1: amino acid length is 500, molecular weight is 57419 Da (predicted); Q86WT6-2: amino acid length is 341, molecular weight is 39254 Da (predicted); Q86WT6-3: amino acid length is 296, molecular weight is 34033 Da (predicted); Q86WT6-4: amino acid length is 279, molecular weight is 32068 Da (predicted).可发生磷酸化修饰。磷酸化是其核定位所必需的TRIM69 存在 4 个异构体，Q86WT6-1：氨基酸个数为 500 个，分子量为 57419 Da (预测)；Q86WT6-2：氨基酸个数为 341 个，分子量为 39254 Da (预测)；Q86WT6-3：氨基酸个数为 296 个，分子量为 34033 Da (预测)；Q86WT6-4：氨基酸个数为 279 个，分子量为 32068 Da (预测)。Phosphorylated. Phosphorylation is necessary for nuclear localization
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Subunit
Homo-multimer; required for antiviral activity.
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SwissProt ID
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Synonyms
RNF36, HSD-34, HSD34, TRIM69, E3 ubiquitin-protein ligase TRIM69, RFP-like domain-containing protein trimless, RING finger protein 36, RING-type E3 ubiquitin transferase TRIM69, Tripartite motif-containing protein 69
Documentation
[1]. Han Y, et al. Characterisation of human RING finger protein TRIM69, a novel testis E3 ubiquitin ligase and its subcellular localisation. Biochem Biophys Res Commun. 2012 Dec 7;429(1-2):6-11. [Content Brief]
[2]. Wang K, et al. Interferon-stimulated TRIM69 interrupts dengue virus replication by ubiquitinating viral nonstructural protein 3. PLoS Pathog. 2018 Aug;14(8):e1007287. [Content Brief]
[3]. Rihn SJ, et al. TRIM69 Inhibits Vesicular Stomatitis Indiana Virus. J Virol. 2019 Oct 15;93(20):. [Content Brief]
[4]. Kueck T, et al. Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State. J Virol. 2019 Dec 15;93(24):. [Content Brief]
[5]. Rong X, et al. TRIM69 inhibits cataractogenesis by negatively regulating p53. Redox Biol. 2019 Apr;22:101157. [Content Brief]
[6]. Song Y, et al. Trim69 is a microtubule regulator that acts as a pantropic viral inhibitor. Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2211467119. [Content Brief]
[7]. Wang Y, et al. The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation. Nucleic Acids Res. 2023 Oct 27;51(19):10568-10589. [Content Brief]
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