VAV3 Antibody (YA2515)

Cat. No.: HY-P82770: Data Sheet COA
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User Guide for Antibodies
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VAV3 Antibody (YA2515) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VAV3.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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VAV3 Antibody (YA2515) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
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VAV3 Antibody (YA2515) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VAV3.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 98 kDa;

Observed band size: 98 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9UKW4

Gene ID

10451 [NCBI]

Immunogen

A synthesized peptide derived from human VAV3 aa750-800/847.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg) using VAV3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from Jurkat(lane 2(20ug)，Hela(lane 3(20ug) using VAV3 Antibody (HY-P82770) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion (By similarity)

Expression：
Tissue_specificity:Isoform 1 and isoform 3 are widely expressed; both are expressed at very low levels in skeletal muscle. In keratinocytes, isoform 1 is less abundant than isoform 3. Isoform 3 is detected at very low levels, if any, in adrenal gland, bone marrow, spleen, fetal brain and spinal cord; in these tissues, isoform 1 is readily detectable

Induction:Down-regulated by EGF and TGF-beta

Isoforms & Post-Translational Modification：Q9UKW4 has 4 isomers: Q9UKW4-1: 97776 Da (predicted); Q9UKW4-2: 86696 Da (predicted); Q9UKW4-3: 32604 Da (predicted); Q9UKW4-4: 100828 Da (predicted).
Phosphorylated. Phosphorylation can be mediated by ROS1. In osteoclasts, undergoes tyrosine phosphorylation in response to CSF1 (By similarity)

Subunit：Interacts with the PH domain of SH2B2. Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with ROS1; constitutive interaction that mediates VAV3 phosphorylation

RRID

AB_3104866

Database

Entrez Gene: 10451 Human ; 57257 Mouse ;

SwissProt: Q9UKW4 Human ; Q9R0C8 Mouse ;

OMIM: 605541 Human

Research Field

Signal Transduction

Synonyms

RGD1565941; VAV 3; Vav3; VAV3 oncogene

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

VAV3 Antibody (YA2515) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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VAV3 Antibody (YA2515)

VAV3 Antibody (YA2515) Featured Recommendations:

Product Detail

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Background

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VAV3 Antibody (YA2515) Related Classifications

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SAFETY DATA SHEET (SDS)

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