Band 3 Antibody (YA1819)

Cat. No.: HY-P82074: Data Sheet COA
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Band 3 Antibody (YA1819) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Band 3.

For research use only. We do not sell to patients.

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10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
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Band 3 Antibody (YA1819) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Band 3 Antibody (YA1819) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Band 3.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 102 kDa;

Observed band size: 102 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P02730

Gene ID

6521 [NCBI]

Immunogen

A synthesized peptide derived from human Band 3 / CD233 aa20-70/911.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB ICC

Western blot analysis of extracts from HEK293(lane 2(20ug) , NIH/3T3(lane 3(20ug) and K562(lane 4(20ug) using Band 3 Antibody (HY-P82074) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Band 3 with Band 3 Antibody (HY-P82074) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Band 3 Antibody (HY-P82074) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling Band 3 with Band 3 Antibody (HY-P82074) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Band 3 Antibody (HY-P82074) at 1/100 at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein (PubMed:10926824, PubMed:14734552, PubMed:1538405, PubMed:16227998, PubMed:20151848, PubMed:24121512, PubMed:28387307, PubMed:35835865). Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin (PubMed:1538405, PubMed:20151848, PubMed:35835865). Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine (PubMed:10926824, PubMed:14734552, PubMed:16227998, PubMed:24121512, PubMed:28387307); (Microbial infection) Acts as a receptor for P.falciparum (isolate 3D7) MSP9 and thus, facilitates merozoite invasion of erythrocytes (PubMed:14630931). Acts as a receptor for P.falciparum (isolate 3D7) MSP1 and thus, facilitates merozoite invasion of erythrocytes (PubMed:12692305)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Detected in erythrocytes (at protein level) ; Expressed in kidney (at protein level)

Isoforms & Post-Translational Modification：P02730 has 2 isomers: P02730-1: 101792 Da (predicted); P02730-2: 94195 Da (predicted).
Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation;Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation;N-glycosylated

Subunit：A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically (PubMed:35835865). Component of the ankyrin-1 complex in the erythrocyte, composed of ANK1, RHCE, RHAG, SLC4A1, EPB42, GYPA, GYPB and AQP1 (PubMed:35835865). Interacts with STOM; this interaction positively regulates SLC4A1 activity (PubMed:23219802, PubMed:28387307). Interacts with GYPA; a GYPA monomer is bound at each end of the SLC4A1 dimer forming a heterotetramer (PubMed:35835865). Three SLC4A1 dimers (Band 3-I, Band 3-II and Band 3-III) participates in the ankyrin-1 complex (PubMed:35835865). Interacts (via the cytoplasmic domain) with EPB42; this interaction is mediated by the SLC4A1 Band 3-I dimer (PubMed:35835865). Interacts (via the cytoplasmic domain) directly with ANK1; this interaction is mediated by the SLC4A1 Band 3-II and Band 3-III dimers (PubMed:35835865, PubMed:7665627)

RRID

AB_3104193

Database

Entrez Gene: 6521 Human

SwissProt: P02730 Human

OMIM: 166900 Human

Research Field

Cardiovascular

Synonyms

AE1; BND3; CD233; DI; EMPB3; EPB3; FR; RTA1A; SLC4A1; SW; WD; WD1; WR

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Band 3 Antibody (YA1819) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Band 3 Antibody (YA1819)

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