ATG13 Antibody (YA7525)

Cat. No.: HY-P87840: Data Sheet User Guide for Antibodies
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ATG13 Antibody (YA7525) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to ATG13.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

ATG13 Antibody (YA7525) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to ATG13.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 56 kDa;

Observed band size: 72 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O75143

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG, Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation

Subcellular Localization：Cytoplasm, cytosol,Preautophagosomal structure

Isoforms & Post-Translational Modification：O75143 has 5 isomers: O75143-1: 56572 Da (predicted); O75143-2: 52823 Da (predicted); O75143-3: 44487 Da (predicted); O75143-4: 44093 Da (predicted); O75143-5: 60155 Da (predicted).
Phosphorylated by ULK1, ULK2 and mTOR

Subunit：Part of a complex consisting of ATG13, ULK1 and RB1CC1 (PubMed:19211835, PubMed:19225151, PubMed:19597335, PubMed:24290141)

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mda-mb-231-cell.html [Content Brief]