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MEIS1 Antibody (YA2438)

(Synonyms: Homeo box protein Meis1; Leukemogenic homolog protein; MEIS 1; Meis homeo box 1; Meis1; Meis1 mouse homolog; MEIS1 protein)
Customer Review

Based on 1 Customer Validation

MEIS1 Antibody (YA2438) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEIS1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MEIS1 Antibody (YA2438)
  • Experimental Validation Results for MEIS1 Antibody (YA2438)
  • MEIS1 Antibody (YA2438) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50 1:50-1:100

Product Details

Description

MEIS1 Antibody (YA2438) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEIS1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human MEIS1 aa320-390/390.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104793

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MEIS1 Antibody (YA2438)
    Western blot analysis was performed on extracts from IMR-32 (lane 1, 20 μg), HeLa (lane 2, 20 μg), K562 (lane 3, 20 μg), SH-SY5Y (lane 4, 20 μg), and Mouse brain (lane 5, 20 μg) using MEIS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MEIS1 Antibody (YA2438)
    Flow cytometric analysis of 1X106 HeLa cells labeling MEIS1 Antibody (HY-P82693, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    MEIS1 acts as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Expressed at low level in normal immunohepatopoietic tissues, including the fetal liver. Expressed in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. Also expressed at high levels in the cerebellum

  • Isoforms & Post-Translational Modification

    O00470 has 2 isomers: O00470-1: 43016 Da (predicted); O00470-2: 50576 Da (predicted).

  • Subunit

    Interacts with the N-terminal region of PBX1 to form a heterodimer which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterodimers with PBX2. Forms heterotrimers with PBX1 or PBX2 and a number of HOX proteins including HOXA9, HOXD4 and HOXD9 where it acts as a non-DNA-binding partner. Also forms heterotrimers with PBX1 and HOX proteins including HOXD9 and HOXD10 where PBX1 is the non-DNA-binding partner. Heterodimer with DLX3 (PubMed:26550823). Heterodimer with HOXB13 (PubMed:28473536)

  • SwissProt ID

    O00470

  • Gene ID
    4211 [NCBI]
  • Synonyms

    Homeo box protein Meis1; Leukemogenic homolog protein; MEIS 1; Meis homeo box 1; Meis1; Meis1 mouse homolog; MEIS1 protein

  • Research Field

    Epigenetics and Nuclear Signaling

MEIS1 Antibody (YA2438) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from IMR-32 (lane 1, 20 μg), HeLa (lane 2, 20 μg), K562 (lane 3, 20 μg), SH-SY5Y (lane 4, 20 μg), and Mouse brain (lane 5, 20 μg) using MEIS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling MEIS1 Antibody (HY-P82693, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MEIS1 Antibody (YA2438) Product Image
    1/16
  • Experimental Validation Results for MEIS1 Antibody (YA2438)
  • Experimental Validation Results for MEIS1 Antibody (YA2438)
  • MEIS1 Antibody (YA2438) Product Image
  • Experimental Validation Results for MEIS1 Antibody (YA2438)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from IMR-32 (lane 1, 20 μg), HeLa (lane 2, 20 μg), K562 (lane 3, 20 μg), SH-SY5Y (lane 4, 20 μg), and Mouse brain (lane 5, 20 μg) using MEIS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MEIS1 Antibody (YA2438)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling MEIS1 Antibody (HY-P82693, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MEIS1 Antibody (YA2438)
  • Experimental Validation Results for MEIS1 Antibody (YA2438)
  • MEIS1 Antibody (YA2438) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from IMR-32 (lane 1, 20 μg), HeLa (lane 2, 20 μg), K562 (lane 3, 20 μg), SH-SY5Y (lane 4, 20 μg), and Mouse brain (lane 5, 20 μg) using MEIS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MEIS1 Antibody (YA2438) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling MEIS1 Antibody (HY-P82693, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MEIS1 Antibody (YA2438) Product Image WB
  • MEIS1 Antibody (YA2438) Product Image FC