RuvB Like 1 Antibody
(Synonyms: 49 kDa TATA box binding protein interacting protein, 49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP-54, ECP54, ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD, INO80 complex subunit H, NMP 238)RuvB Like 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to RuvB Like 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:50000 | 1:100 | 1:5000 | 1:5000 |
Product Details
RuvB Like 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to RuvB Like 1.
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Host Rabbit
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Clonality polyclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 50 kDa;
Recombinant protein of Human TIP49A (aa 121-320).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RuvB Like 1 is a Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation. May be able to bind plasminogen at cell surface and enhance plasminogen activation[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Nucleus matrix; Nucleus, nucleoplasm; Cytoplasm; Membrane; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Dynein axonemal particle
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Expression
Tissue_Specificity: Ubiquitously expressed with high expression in heart, skeletal muscle and testis. -
Isoforms & Post-Translational Modification
RuvB Like 1 has 2 isoforms, Q9Y265-1: amino acid length is 456, molecular weight is 50228 Da (predicted); Q9Y265-2: amino acid length is 386, molecular weight is 42127 Da (predicted).
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Subunit
Forms homohexameric rings.
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SwissProt ID
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Synonyms
49 kDa TATA box binding protein interacting protein, 49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP-54, ECP54, ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD, INO80 complex subunit H, NMP 238
Documentation
[1]. Puri T, et al. Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. J Mol Biol. 2007 Feb 9;366(1):179-92. [Content Brief]
[2]. López-Perrote A, et al. Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. Elife. 2020 Nov 18;9:. [Content Brief]
[3]. Doyon Y, et al. Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans. Mol Cell Biol. 2004 Mar;24(5):1884-96. [Content Brief]
[4]. Obri A, et al. ANP32E is a histone chaperone that removes H2A.Z from chromatin. Nature. 2014 Jan 30;505(7485):648-53. [Content Brief]
[5]. Jin J, et al. A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex. J Biol Chem. 2005 Dec 16;280(50):41207-12. [Content Brief]
[6]. Chen L, et al. Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. J Biol Chem. 2011 Apr 1;286(13):11283-9. [Content Brief]
[7]. Wood MA, et al. An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc. Mol Cell. 2000 Feb;5(2):321-30. [Content Brief]
[8]. Weiske J, et al. The histidine triad protein Hint1 interacts with Pontin and Reptin and inhibits TCF-beta-catenin-mediated transcription. J Cell Sci. 2005 Jul 15;118(Pt 14):3117-29. [Content Brief]
[9]. Gartner W, et al. The ATP-dependent helicase RUVBL1/TIP49a associates with tubulin during mitosis. Cell Motil Cytoskeleton. 2003 Oct;56(2):79-93. [Content Brief]
[10]. Hawley SB, et al. Purification, cloning, and characterization of a profibrinolytic plasminogen-binding protein, TIP49a. J Biol Chem. 2001 Jan 5;276(1):179-86. [Content Brief]
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