BMP-2 Antibody

Cat. No.: HY-P81093: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

BMP-2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to BMP-2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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BMP-2 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

BMP-2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to BMP-2.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat Predicted Reactivity: Chicken, Dog, Cow, Horse, Rabbit, Sheep

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P12643

Gene ID

650 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human BMP2: 283-365/396

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from M-Spleen using BMP2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:24362451, PubMed:31019025). Induces cartilage and bone formation (PubMed:3201241). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 (PubMed:31800957). Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:16771708, PubMed:20851880). Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity)

Subcellular Localization：Secreted

Expression：
Tissue_specificity:It is particularly abundant in the lungs, spleen, and colon, and present in lower but significant amounts in the heart, brain, placenta, liver, skeletal muscle, kidneys, pancreas, prostate, ovaries, and small intestine.

Subunit：Homodimer; disulfide-linked (PubMed:10074410). Interacts with SOSTDC1 (PubMed:15020244). Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN (By similarity). Interacts with MAFP5 (By similarity). Interacts with FBN1 (via N-terminal domain) and FBN2 (PubMed:18339631). Interacts with type I receptor BMPR1A (PubMed:15064755). Interacts with type II receptor BMPR2 (PubMed:7791754). Interacts with SCUBE3 (PubMed:33308444). Interacts with TNFAIP6 (primarily via Link domain); this interaction is inhibited by hyaluronan. Interacts with ERFE (PubMed:31800957). Interacts with BMPR1A/ALK3; the interaction may induce HAMP expression (PubMed:31800957). Forms heterodimers with BMP6 in vitro; the heterodimer then binds to its receptor BMPR1A /ALK3 and may induce HAMP expression (PubMed:31800957). Interacts with TGFBR3 (PubMed:18184661)

RRID

AB_3103204

Database

Entrez Gene: 650 Human ; 12156 Mouse ; 29373 Rat

SwissProt: P12643 Human ; P21274 Mouse ; P49001 Rat

OMIM: 112600 Human

Synonyms

Bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2A; BMP-2; BMP2_HUMAN.

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

BMP-2 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BMP-2 Antibody

BMP-2 Antibody Featured Recommendations:

Product Detail

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Background

Documentation

BMP-2 Antibody Related Classifications

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