EPO Receptor Antibody
(Synonyms: Erythropoietin receptor, EPO-R, EPOR)EPO Receptor Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to EPO Receptor.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
EPO Receptor Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to EPO Receptor.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 26; 35; 55 kDa
Purified recombinant protein of human EPO Receptor.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
EPO Receptor is a Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase (By similarity)[1][2][3][4][5][6].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Erythroid cells and erythroid progenitor cells. -
Isoforms & Post-Translational Modification
EPO Receptor has 3 isoforms, P19235-1: amino acid length is 508, molecular weight is 55065 Da (predicted); P19235-2: amino acid length is 241, molecular weight is 26456 Da (predicted); P19235-3: amino acid length is 328, molecular weight is 35809 Da (predicted).
On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site. -
Subunit
Forms homodimers on EPO stimulation.
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SwissProt ID
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Synonyms
Erythropoietin receptor, EPO-R, EPOR
Documentation
[1]. Zhan H, et al. Engineering a soluble extracellular erythropoietin receptor (EPObp) in Pichia pastoris to eliminate microheterogeneity, and its complex with erythropoietin. Protein Eng. 1999 Jun;12(6):505-13. [Content Brief]
[2]. Winkelmann JC, et al. The gene for the human erythropoietin receptor: analysis of the coding sequence and assignment to chromosome 19p. Blood. 1990 Jul 1;76(1):24-30. [Content Brief]
[3]. Jones SS, et al. Human erythropoietin receptor: cloning, expression, and biologic characterization. Blood. 1990 Jul 1;76(1):31-5. [Content Brief]
[4]. Middleton SA, et al. Identification of a critical ligand binding determinant of the human erythropoietin receptor. Evidence for common ligand binding motifs in the cytokine receptor family. J Biol Chem. 1996 Jun 14;271(24):14045-54. [Content Brief]
[5]. Syed RS, et al. Efficiency of signalling through cytokine receptors depends critically on receptor orientation. Nature. 1998 Oct 1;395(6701):511-6. [Content Brief]
[6]. Kirito K, et al. Identification of the human erythropoietin receptor region required for Stat1 and Stat3 activation. Blood. 2002 Jan 1;99(1):102-10. [Content Brief]
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