Beta Tubulin Antibody (HRP) (YA865)

Cat. No.: HY-P80955A: Data Sheet COA
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beta Tubulin Antibody (HRP) (YA865) is a HRP-conjugated, mouse-derived, anti-beta Tubulin (HRP) (YA865) IgG1 monoclonal antibody.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Beta Tubulin Antibody (HRP) (YA865) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

beta Tubulin Antibody (HRP) (YA865) is a HRP-conjugated, mouse-derived, anti-beta Tubulin (HRP) (YA865) IgG1 monoclonal antibody.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 50 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey, Hamster, Rat

SwissProt ID

P07437

Gene ID

203068 [NCBI]

Immunogen

Purified recombinant human Tubulin beta protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000-1:10000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	HRP	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), K562 (lane 2, 20 μg), 3T3 (lane 3, 20 μg) , COS-7 (lane 4, 20 μg), C6 (lane 5, 20 μg), and CHO-K1 (lane 6, 20 μg) using beta Tubulin Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:5000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin

Subcellular Localization：Cytoplasm, cytoskeleton

Expression：
Tissue_specificity:This gene is widely expressed in all tissues of the body, with the highest expression levels in the spleen, thymus, and immature brain tissue.

Induction:Autoregulated by feedback control of mRNA degradation (PubMed:31727855) . In excess of soluble tubulin, nascent beta-tubulin chain binds TTC5/STRAP cofactor through the MREI motif which triggers cotranslation degradation of tubulin mRNA (PubMed:31727855)

Subunit：Heterodimer of alpha and beta chains (PubMed:26637975). A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with CIMAP3 (PubMed:20643351). Interacts with DIAPH1 (PubMed:23325789). Interacts with MX1 (By similarity). May interact with RNABP10 (By similarity). Interacts with CFAP157 (By similarity). Nascent tubulin polypeptide interacts (via beta-tubulin MREI motif) with TTC5/STRAP; this interaction results in tubulin mRNA-targeted degradation (PubMed:31727855)

RRID

AB_3103149

Database

Entrez Gene: 203068 Human ; 22154 Mouse ;

SwissProt: P07437 Human ; P99024 Mouse ;

OMIM: 615771 Human

Research Field

Cell Biology

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Beta Tubulin Antibody (HRP) (YA865) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Beta Tubulin Antibody (HRP) (YA865)

Beta Tubulin Antibody (HRP) (YA865) Featured Recommendations:

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