SIRT6 Antibody (YA5214)
(Synonyms: 2810449N18Rik; AI043036; Mono ADP ribosyltransferase sirtuin 6; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; Regulatory protein SIR2 homolog; SIR2 like 6; SIR2 like protein 6; Sir2 related protein type 6; SIR2-like protein 6; SIR2)SIRT6 Antibody (YA5214) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SIRT6.
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Host:
Mouse
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Application:
WB, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat, Monkey
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IF
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|---|---|---|
| Dilution Ratio | 1:500 | ICC/IF: 1:100 |
Product Details
SIRT6 Antibody (YA5214) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SIRT6.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat, Monkey
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Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Purified recombinant human SIRT6 protein expressed in E.coli.
affinity chromatography.
Non-conjugated
Unmodified
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SIRT6 is a nuclear NAD+-dependent sirtuin that functions as a deacetylase, deacylase, and mono-ADP-ribosyltransferase, thereby maintaining chromatin integrity, DNA repair capacity, telomere stability, and metabolic homeostasis[1][2]. Mechanistically, SIRT6 regulates multiple signaling and transcriptional programs involved in aging, cellular stress responses, glucose and lipid metabolism, inflammation, and genome maintenance, placing it at the intersection of epigenetic regulation and cellular adaptation[2][5][3]. Through its effects on DNA damage repair, chromosome maintenance, and metabolic control, SIRT6 contributes to the preservation of cellular function and resistance to age-associated dysfunction[5][3]. In disease contexts, altered SIRT6 activity has been linked to cancer, cardiovascular disease, diabetes, and neurodegenerative disorders, while experimental studies demonstrate that SIRT6 deficiency induces mitochondrial dysfunction, oxidative stress, and broad transcriptional changes associated with brain aging and neurodegeneration[2][4]. Compared with related sirtuin isoforms, SIRT6 is distinguished by its predominant chromatin-associated functions and its combined deacetylase, deacylase, and mono-ADP-ribosyltransferase activities that directly couple NAD+ sensing to epigenetic and genome-stability pathways[1][5]. For experimental applications, increasing attention has focused on small-molecule SIRT6 modulators, including both activators and inhibitors, which provide useful tools for mechanistic studies and evaluation of SIRT6-dependent pathways in aging, metabolism, cancer, and neurodegenerative disease models[2][6].
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Subcellular Localization
Nucleus; Chromosome; Chromosome, telomere; Endoplasmic reticulum
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Expression
Induction:Down-regulated in a number of cancers, such as pancreatic cancer or colon carcinomas (PubMed:23217706) . Post-transcriptionally regulated by miR-766 (PubMed:23653361) . Expression is post-transcriptionally repressed by miR-122 (PubMed:26748705) -
Isoforms & Post-Translational Modification
Q8N6T7 has 2 isomers: Q8N6T7-1: 39119 Da (predicted); Q8N6T7-2: 36065 Da (predicted).
Acetylated at Lys-33 (PubMed:32538779). Deacetylation at Lys-33 by SIRT1 promotes homomultimerization and binding to double-strand breaks (DSBs) sites (PubMed:32538779);Phosphorylation at Ser-10 by MAPK8/JNK1 in response to oxidative stress stimulates the mono-ADP-ribosyltransferase activity on PARP1, leading to PARP1 recruitment to double-strand breaks (DSBs);Monoubiquitinated at Lys-170 by STUB1/CHIP, preventing its degradation by the proteasome. Deubiquitinated by USP10, also preventing its degradation by the proteasome (PubMed:37023208);Sumoylated, leading to specifically decrease ability to deacetylate histone H3 at 'Lys-56' (H3K56ac) -
Subunit
Homodimer; binds to nucleosomes and DNA ends as a homodimer (PubMed:31995034, PubMed:32538779). Interacts with RELA; interferes with RELA binding to target DNA (PubMed:19135889). Interacts with SMARCA5; promoting recruitment of SMARCA5/SNF2H to double-strand breaks (DSBs) sites (PubMed:23911928). Interacts with the mTORC2 complex; preventing the ability of SIRT6 to deacetylate FOXO1. Interacts with the CLOCK-BMAL1 complex; recruited by the CLOCK-BMAL1 complex to regulate expression of clock-controlled genes. Interacts with CSNK2A2; preventing CSNK2A2 localization to the nucleus (By similarity)
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SwissProt ID
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Synonyms
2810449N18Rik; AI043036; Mono ADP ribosyltransferase sirtuin 6; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; Regulatory protein SIR2 homolog; SIR2 like 6; SIR2 like protein 6; Sir2 related protein type 6; SIR2-like protein 6; SIR2
Documentation
References
[1]. Uniprotkb.
[2]. Chen RR, et al. SIRT6 in health and diseases: From molecular mechanisms to therapeutic prospects. Pharmacol Res. 2025 Nov;221:107984. [Content Brief]
[3]. SIRT6 gene information from NCBI.
[4]. Smirnov D, et al. SIRT6 is a key regulator of mitochondrial function in the brain. Cell Death Dis. 2023 Jan 18;14(1):35. [Content Brief]
[5]. Li Y, et al. SIRT6 Widely Regulates Aging, Immunity, and Cancer. Front Oncol. 2022;12:861334.
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