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KDM5B Antibody (YA6884)

(Synonyms: Cancer/testis antigen 31 antibody)
Customer Review

Based on 1 Customer Validation

KDM5B Antibody (YA6884) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KDM5B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KDM5B Antibody (YA6884)
  • KDM5B Antibody (YA6884) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1-2μg/sample

Product Details

Description

KDM5B Antibody (YA6884) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KDM5B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 145 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size：176 kDa
Immunogen

Synthetic peptide within Human KDM5B aa 960-1,009 / 1,544.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KDM5B Antibody (YA6884)
    Western blot analysis of extracts from A549 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and HepG2 (lane 3(20μg)) using KDM5B Antibody (HY-P87196) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    KDM5B is a Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2[1][2][3].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_Specificity: Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level)

  • Isoforms & Post-Translational Modification

    Q9UGL1 has two isomers: Q9UGL1-1: 175658 Da (predicted); Q9UGL1-2: 179409 Da (predicted).

  • Subunit

    Interacts with FOXG1B, PAX9, MYC, MYCN and RB1

  • SwissProt ID

    Q9UGL1

  • Synonyms

    Cancer/testis antigen 31 antibody

KDM5B Antibody (YA6884) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and HepG2 (lane 3(20μg)) using KDM5B Antibody (HY-P87196) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • KDM5B Antibody (YA6884) Product Image
    1/16
  • Experimental Validation Results for KDM5B Antibody (YA6884)
  • KDM5B Antibody (YA6884) Product Image
  • Experimental Validation Results for KDM5B Antibody (YA6884)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and HepG2 (lane 3(20μg)) using KDM5B Antibody (HY-P87196) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KDM5B Antibody (YA6884)
  • KDM5B Antibody (YA6884) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and HepG2 (lane 3(20μg)) using KDM5B Antibody (HY-P87196) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • KDM5B Antibody (YA6884) Product Image WB