2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. Cytokeratin 19 Antibody (YA924)

Cytokeratin 19 Antibody (YA924)

Cat. No.: HY-P81250
User Guide for Antibodies Technical Support

Cytokeratin 19 Antibody (YA924) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 19.

For research use only. We do not sell to patients.

Size Price Stock Quantity
20 μL Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks
50 μL Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks
100 μL Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks
250 μL   Get quote  
Synthetic products have potential research and development risk.
or Bulk Inquiry

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

Description

Cytokeratin 19 Antibody (YA924) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 19.
Host

Mouse
Clonality

Monoclonal
Molecular Weight
Predicted band size: 44 kDa;
Observed band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human Predicted Reactivity: Mouse
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
SwissProt ID
Gene ID
Immunogen

KLH conjugated synthetic peptide derived from human Cytokeratin 19.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-500
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:100-500
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-500
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
ICC

  • Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81250) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81250) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P81013) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81250) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81250) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P81013) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Background
Function：Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle
Expression：
Tissue_specificity:It is expressed in specific basal keratinocyte regions of the deep outer root sheath of hair follicles. It is also found in sweat glands and mammary ducts and secretory cells, bile ducts, gastrointestinal tract, bladder urothelial tissue, oral epithelium, esophagus, and cervical external epithelium (protein level) . It is expressed in specific regions of epidermal basal cells, nipple epidermis, and hair follicles. It is also found in some wall cells of the human umbilical vein and arteries, as well as in the smooth muscle of umbilical vessels. In structures containing dystrophin and spectroscopylin, muscle fibers aggregate in sarcomeres within the sarcoplasm of the sarcolemma.
Subunit：Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein
Database

Entrez Gene: 3880 Human

SwissProt: P08727 Human

OMIM: 148020 Human

Synonyms
40 kDa keratin intermediate filament; CK 19; ck19; CK19; CK-19; KRT19; Cytokeratin19; Cytokeratin-19; k19; k1cs; Keratin 19; Keratin-19; Keratin type I 40 kD; Keratin type i 40kD; Keratin type I cytoskeletal 19; krt19; mgc15366; K1C19_HUMAN; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd
Documentation
User Guide for Antibodies (1077 KB)

Cytokeratin 19 Antibody (YA924) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Cytokeratin 19 Antibody (YA924)
Cat. No.:
HY-P81250
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.