Hexokinase I Antibody (YA4901)

Cat. No.: HY-P85209: Data Sheet User Guide for Antibodies
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Hexokinase I Antibody (YA4901) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Hexokinase I.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Hexokinase I Antibody (YA4901) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Hexokinase I.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 102 kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P19367

Gene ID

3098 [NCBI]

Immunogen

Purified recombinant fragment of human HXK I expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331)

Subcellular Localization：Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasm, cytosol

Expression：
Tissue_specificity:Isotype 2: Erythrocyte specific (Reference 6) . Isotype 3: Testis specific (PubMed: 10978502) . Isotype 4: Testis specific (PubMed: 10978502) .

Isoforms & Post-Translational Modification：P19367 has 4 isomers: P19367-1: 102486 Da (predicted); P19367-2: 102201 Da (predicted); P19367-3: 102738 Da (predicted); P19367-4: 101085 Da (predicted).

Subunit：Monomer (PubMed:10686099). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with VDAC1 (PubMed:22304920). The HK1-VDAC1 complex interacts with ATF2 (PubMed:22304920). Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity). Interacts with SMAD5 (PubMed:28675158)

Synonyms

HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

Documentation

User Guide for Antibodies (1077 KB)