Hexokinase I Antibody (YA4901)
(Synonyms: HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I)Hexokinase I Antibody (YA4901) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Hexokinase I.
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Host:
Mouse
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Application:
WB, IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Hexokinase I Antibody (YA4901) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Hexokinase I.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 102 kDa;
Purified recombinant fragment of human HXK I expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Hexokinase I catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome[1][2][3].
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Subcellular Localization
Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasm, cytosol
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Expression
Tissue_specificity:Isotype 2: Erythrocyte specific (Reference 6) . Isotype 3: Testis specific (PubMed: 10978502) . Isotype 4: Testis specific (PubMed: 10978502) . -
Isoforms & Post-Translational Modification
P19367 has 4 isomers: P19367-1: 102486 Da (predicted); P19367-2: 102201 Da (predicted); P19367-3: 102738 Da (predicted); P19367-4: 101085 Da (predicted).
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Subunit
Monomer (PubMed:10686099). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with VDAC1 (PubMed:22304920). The HK1-VDAC1 complex interacts with ATF2 (PubMed:22304920). Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity). Interacts with SMAD5 (PubMed:28675158)
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SwissProt ID
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Synonyms
HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I
Documentation
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